| Rudimentary study on humanization of porcine red blood cells:Enzymatic removal of galactose-α1,3-galactose antigen from porcine red blood cell |
| |
| 作者姓名： | WANGJiexi BAIYan |
| |
| 作者单位： | [1]InstituteofBloodTransfusion,AcademyofMilitaryMedicalSceinces,Beijing100850,China [2]DepartmentofHematology,The307Hospital,Beijing100039,China |
| |
| 摘 要： | The serological and biochemical characterization of porcine red blood cells (pRBCs) are similar to human red blood cells,Porcine erythrocytes are considered as an alternative source for human blood transfusion.But there exist galactose-α1,3-galactose antigens (Galα1,3Galβ1,4galNAc-R,abbreviated αGal antigen ) on pRBCs,which can induce anti-αGai antibodies in human serum ,The αGal epitopes are the major antigen responsible for hyperacute rejection in exnotransfusion .In this study ,recombined soybean α-galactosidase (rSα-GalE) was used to remove the αGal antigens from pPRCs for humanization .The results showed that αGal antigen was eleared by rSα-GalE and the structure and function of rSα-GalE treated pRBC were normal.
|
| 关 键 词： | 猪红细胞 半乳糖-α1 3-半乳糖抗原 酶法脱除 重组大豆半乳糖苷酶 人血化 猪血 |
| 收稿时间： | 2002-10-21 |
Rudimentary study on humanization of porcine red blood cells: Enzymatic removal of galactose-α1,3-galactose antigen from porcine red blood cell |
| |
| WANGJiexi BAIYan.Rudimentary study on humanization of porcine red blood cells: Enzymatic removal of galactose-α1,3-galactose antigen from porcine red blood cell[J].Chinese Science Bulletin,2003,48(4):343-345. |
| |
| Authors: | Wang Jiexi Gao Xin Bai Yan Ren Huiming Tan Yingxia Zhang Yangpei |
| |
| Institution: | WANG Jiexi1, GAO Xin1, BAI Yan2, REN Huiming1, TAN Yingxia1 & ZHANG Yangpei1 1. Institute of Blood Transfusion, Academy of Military Medical Sciences, Beijing 100850, China; 2. Department of Hematology, The 307 Hospital, Beijing 10039, China Correspondence should be addressed to Zhang Yangpei |
| |
| Abstract: | The serological and biochemical characterization of porcine red blood cells (pRBCs) are similar to human red blood cells.
Porcine erythrocytes are considered as an alternative source for human blood transfusion. But there exist galactose-α1,3-galactose
antigens (Galα1,3Galβ1, 4GalNAc-R, abbreviated αGal antigen) on pRBCs, which can induce anti-αGal antibodies in human serum.
The αGal epitopes are the major antigen responsible for hyperacute rejection in xenotransfusion. In this study, recombined
soybean α-galactosidase (rSα-GalE) was used to remove the αGal antigens from pPRCs for humanization. The results showed that
αGal antigen was cleared by rSα-GalE and the structure and function of rSα-GalE treated pRBC were normal. |
| |
| Keywords: | porcine red blood cell galactose-α1 3-galactose antigen recombined soybean α-galactosidase |
| 本文献已被 CNKI 维普 万方数据 SpringerLink 等数据库收录！ |
| 点击此处可从《中国科学通报(英文版)》浏览原始摘要信息 |
| 点击此处可从《中国科学通报(英文版)》下载免费的PDF全文 |
|
