短小芽孢杆菌LYMC-3菌株对拟茎点霉的拮抗作用

【目的】短小芽孢杆菌LYMC-3菌株对稻瘟病、黄瓜枯萎病及棉花黄萎病等具有显著的防治效果,测定LYMC-3菌株对8种植物病原真菌的抑菌活性,检测其抑菌谱,为其推广应用提供依据。【方法】采用平板对峙法测定LYMC-3菌株对松球壳孢菌(Sphaeropsis sapinea)、松树脂溃疡病菌(Fusarium circinatum)、金黄壳囊孢菌(Cytospora chrysosperma)、拟茎点霉(Phomopsis macrospora)、七叶树壳梭孢菌(Fusicoccum aesculi)、拟盘多毛孢(Pestalotiopsis theae)、山茶炭疽菌(Guignardia camelliae)、葡萄座腔菌(Botryosphaeria dothiorella)8种植物病原真菌的抑菌活性,筛选出抑菌活性最强的病原真菌,进行菌丝生长和孢子萌发抑制试验。【结果】LYMC-3菌株对8种供试植物病原真菌均有抑制作用,抑菌带长度及抑菌活性大小依次为:拟茎点霉>拟盘多毛孢>葡萄座腔菌>松球壳孢菌>七叶树壳梭孢菌>金黄壳囊孢菌>松树脂溃疡病菌>山茶炭疽菌。LYMC-3菌株发酵滤液对拟茎点霉菌丝生长的抑制率为70.36%,对其分生孢子24 h萌发抑制率为88.16%。【结论】短小芽孢杆菌LYMC-3菌株抑菌谱广,其发酵滤液对拟茎点霉有较强的拮抗作用。

The antagonism of Bacillus pumilus LYMC-3 strain on Phomopsis macrospora

【Objective】The Bacillus pumilus LYMC-3 strain has a considerable control effect on the rice blast, cucumber fusarium wilt, and cotton verticillium wilt. To further research on the biocontrol potential of this strain, the antibacterial spectrum was tested by measuring the antibacterial activity of LYMC-3 strain against eight types of plant pathogenic fungi to provide a reference for its subsequent popularization and application.【Method】The antibacterial activity of LYMC-3 strain against eight plant pathogenic fungi (Sphaeropsis sapinea, Fusarium circinatum, Cytospora chrysosperma, Phomopsis macrospora, Fusicoccum aesculi, Pestalotiopsis theae, Guignardia camelliae and Botryosphaeria dothiorella) was determined using the plate pairing method, and one of them was selected for the mycelial growth and spore germination inhibition tests.【Result】The LYMC-3 strain had a wide antimicrobial spectrum, with inhibitory effects on many plant pathogenic fungi that were tested. The length of the inhibitory zone and the magnitude of the antibacterial activity were in the following order: Phomopsis macrospora > Pestalotiopsis theae > Botryosphaeria dothiorella > Sphaeropsis sapinea > Fusicoccum aesculi > Cytospora chrysosperma > Fusarium circinatum > Guignardia camelliae. The inhibition rate of the fermentation broth of the LYMC-3 strain to the mycelial growth of Phomopsis macrospora was 70.36%, and the spore germination in 24 hours was 88.16%.【Conclusion】The Bacillus pumilus LYMC-3 strain has a wide antimicrobial spectrum. The fermentation filtrate broth of the strain has a high antagonistic effect on Phomopsis macrospora.