Study on the intermediate in theo-phenylenediamine oxidative reaction using hemoglobin as a mimetic peroxidase in aqueous-organic two phase |
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Authors: | Li Hai-cheng Li De-jia Huang Bo Jin De-long Zou Guo-lin |
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Institution: | (1) College of Life Sciences, Wuhan University, 430072 Wuhan, Hubei, China |
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Abstract: | Hemoglobin was used as a mimetic enzyme for peroxidase to catalyze the oxidative reaction of o-phenylenediamine with H2O2 which functioned as an oxidant. The relationship between physicochemical properties of the intermediate and enzymatic activity
of hemoglobin was studied. Since the solubility of the intermediate in the reaction is higher in butanol phase than in water
phase, the intermediate itself diffused from the aqueous phase to the butanol phase. The experimental results showed that
the rate of product and the stability of intermediate were associated with the temperature and the pH value of the buffer.
The formation rate of intermediate and half-life period reveal the maximal in pH7, nevertheless, the whole rate of the catalytic
reaction is the greatest in pH5, which the ratio of the initial rate in final product formation compared to that intermediate
formation is the greatest.
Foundation item: Supported by the National Natural Science Foundation of China (No. 39770200)
Biography: Li Hai-cheng (1960-), male, Ph. D candidate, Pyongyang, D. P. R. Korea, research direction; mimetic enzyme. |
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Keywords: | hemoglobin mimetic enzyme intermediate two phase reaction mechanism |
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