| 复合诱变选育高产γ-氨基丁酸菌株 |
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| 引用本文： | 刘辉,张庆庆,吕闻闻.复合诱变选育高产γ-氨基丁酸菌株[J].安徽工程科技学院学报,2011,26(3). |
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| 作者姓名： | 刘辉 张庆庆 吕闻闻 |
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| 作者单位： | 安徽工程大学微生物发酵安徽省工程技术研究中心,安徽芜湖,241000 |
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| 基金项目： | 芜湖市重点科技计划基金资助项目 |
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| 摘 要： | 以红曲霉为出发菌株,采用紫外线和5-氟尿嘧啶对其原生质体复合诱变处理,得到一株高产γ-氨基丁酸的菌株ZL307.试验结果表明,经紫外线照射处理150s,以0.3mg/mL的5-氟尿嘧啶处理15min后,γ-氨基丁酸的产量可高达491.1mg/L.与出发菌株相比,产量提高了60%,且高产遗传特性经10次传代仍稳定.
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| 关 键 词： | 红曲霉 γ-氨基丁酸 原生质体 紫外线 5-氟尿嘧啶 |
Compound mutation screening of γ-aminobutyric acid high-yield strain |
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| Abstract: | Monascus was used as the starting strain and the protoplasm underwent UV and 5-fluorouracil complex mutation treatment,and a high-yield γ-aminobutyric acid of Monascus strain ZL307 was obtained.The results showed that after UV irradiation for 150 seconds and 0.3 mg/mL 5-fluorouracil treatment for 15 min,the yield of γ-aminobutyric acid was 491.1 mg/L.The production was improved by 60% compared with that of original strain.The production level of this mutant is stable after 10 continuous generations of passage. |
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| Keywords: | Monascus γ-aminobutyric acid Protoplast UV 5-fluorouracil |
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