| 甲醛与NaOH对污泥EPS中蛋白质和多糖测定的影响 |
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| 引用本文： | 张子健,吴伟伟,王建龙.甲醛与NaOH对污泥EPS中蛋白质和多糖测定的影响[J].清华大学学报(自然科学版),2009(9). |
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| 作者姓名： | 张子健 吴伟伟 王建龙 |
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| 作者单位： | 清华大学核能与新能源技术研究院; |
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| 基金项目： | 国家自然科学基金资助项目(59978020) |
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| 摘 要： | 甲醛-NaOH法广泛应用于污泥胞外多聚物(extracellular polymeric substance,EPS)的提取,但提取过程中残留的化学物质会干扰后续胞外多聚物组分的测定,通过改变甲醛用量和NaOH浓度研究了其对EPS中蛋白质和多糖测定的影响。结果表明:提高甲醛投加量和NaOH浓度,会降低蛋白质测定标线的线性、斜率及数据的精密度。但当甲醛投加量为12~20μL,NaOH浓度为1 mol/L时,蛋白质标线的斜率和线性与不加甲醛和NaOH时相差不大,测试数据有较好的精密度。甲醛投加量与NaOH浓度对多糖测定几乎没有影响,因而在EPS提取之后不需要透析去除残留的甲醛,可直接进行测定。
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| 关 键 词： | 胞外多聚物（EPS） 提取 甲醛 蛋白质 多糖 污泥 |
Influence of formaldehyde and NaOH on the analysis of proteins and polysaccharides in sludge EPS |
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| ZHANG Zijian,WU Weiwei,WANG Jianlong.Influence of formaldehyde and NaOH on the analysis of proteins and polysaccharides in sludge EPS[J].Journal of Tsinghua University(Science and Technology),2009(9). |
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| Authors: | ZHANG Zijian WU Weiwei WANG Jianlong |
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| Institution: | Institute of Nuclear and New Energy Technology;Tsinghua University;Beijing 100084;China |
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| Abstract: | The formaldehyde-NaOH method is widely used in extracellular polymeric substance(EPS) extraction from sludge,but the residual chemicals should be removed since they interfere with subsequent analyses.The influences of formaldehyde dosage and NaOH concentration on the analysis of proteins and polysaccharides for EPS extraction from sludge were investigated.The results show that for the protein analysis,the increased formaldehyde dosage and NaOH concentration increased the analysis uncertainty and reduced the... |
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