| HPLC-QTOFMS和MS/MS分析鉴定PF573228肝微粒体体外代谢产物 |
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| 引用本文： | 王 献,王 玲.HPLC-QTOFMS和MS/MS分析鉴定PF573228肝微粒体体外代谢产物[J].中南民族大学学报(自然科学版),2014(3):9-12. |
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| 作者姓名： | 王 献 王 玲 |
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| 作者单位： | 中南民族大学化学与材料科学学院,武汉,430074 |
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| 基金项目： | 国家自然科学基金资助项目(21275167)；中南民族大学中央专项重点资助项目 |
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| 摘 要： | 为研究PF573228的大鼠肝微粒体体外代谢特点,采用高效液相色谱-电喷雾-串联质谱(HPLC-ESI-MS/MS)的方法,结合检测的保留时间、精确质量数和特征碎片离子,鉴定了PF573228体外的3种代谢产物分子的结构,推断体外代谢产物的生物转化有3个主要途径:脱氢,N-脱烷基和氧化.
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| 关 键 词： | PF573228抑制剂 液质联用(LC-MS/MS) 大鼠肝微粒体 体外代谢 |
Structural Elucidation of in vitro Metabolites of PF573228 by HPLC-QTOF MS and MS/MS |
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| Wang Xian,Wang Ling.Structural Elucidation of in vitro Metabolites of PF573228 by HPLC-QTOF MS and MS/MS[J].Journal of South-Central Univ for,2014(3):9-12. |
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| Authors: | Wang Xian Wang Ling |
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| Institution: | ( College of Chemistry and Materials Science, South-Central University for Nationalities, Wuhan 430074, China ) |
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| Abstract: | To study the in vitro metabolic characteristics of PF573228 with rat liver microsomes, HPLC-ESI-MS/MS was applied and elucidated three metabolites of PF 573228 based on the chromatographic retention times , accurate ion mass and fragmentation pattern .Three biotransformation pathways , i.e., dehydrogenation , N-dealkylation and oxidation metabolism were suggested for the 3 metabolites. |
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| Keywords: | PF573228 inhibitor HPLC-ESI-MS/MS rat liver microsomes in vitro metabolism |
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