| 兔窦房结细胞的分离方法及其起搏电流特征性探讨 |
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| 引用本文： | 王腾,吴平亚,范新荣,陈勇军,陈卉,黄从新.兔窦房结细胞的分离方法及其起搏电流特征性探讨[J].科技导报(北京),2011,29(23):29-32. |
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| 作者姓名： | 王腾 吴平亚 范新荣 陈勇军 陈卉 黄从新 |
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| 作者单位： | 1. 武汉大学人民医院心内科,武汉 430060;2. 武汉市洪山区张家湾社区卫生服务中心,武汉 430070 |
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| 基金项目： | 国家自然科学基金项目(30600337) |
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| 摘 要： | 探讨了家兔窦房结(Sino-Atrial Node,SAN)细胞的分离方法及其形态和起搏电流(Funny Current,If)的特征.通过打结定位法确定了带有"中心结区"的家兔SAN组织条,双酶法分离SAN细胞,利用全细胞穿孔膜片钳技术记录SAN细胞的If.结果显示,SAN组织为上宽下窄的枣核型,区域体积约为6.25mm×2.23mm×0.26mm,或6mm×2mm×0.1mm—6mm×2mm×3.3mm.SAN细胞成狭长梭形,无清晰横纹,细胞长(65.3±7.8)μm,宽为(7.2±3.1)μm.在正常Tyrode液中,可恢复规则自律性搏动,频率为(187.3±5.6)次/min,典型SAN细胞数量占整个分离细胞的2%—5%.根据电压钳制方案,If电流被引出,呈内向性斜坡状,随着钳制电压降低,起搏电流逐渐增大,并随钳制时间延长,倾斜度也增大.If带有明显尾电流,也随着超级化激活电位降低,逐渐增大.根据二者电流大小拟合的电流-电压曲线,呈典型横向Y形.本法获得SAN细胞形态典型,状态和活性良好,能够记录具有特征性的If,可供心血管领域有关针对SAN细胞If研究的技术借鉴.
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| 关 键 词： | 窦房结 细胞形态学 超级化激活起搏电流 |
| 收稿时间： | 2011-06-08 |
Sino-atrial Node Cell Dissociation Method and Pacemaker Current Characteristics in Rabbits |
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| WANG Teng,WU Pingya,FAN Xinrong,CHEN Yongjun,CHEN Hui,HUANG Congxin.Sino-atrial Node Cell Dissociation Method and Pacemaker Current Characteristics in Rabbits[J].Science & Technology Review,2011,29(23):29-32. |
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| Authors: | WANG Teng WU Pingya FAN Xinrong CHEN Yongjun CHEN Hui HUANG Congxin |
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| Institution: | 1. Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan 430060, China;2. Community Health Service Center, Zhangjiangwan Street, Hongshan District, Wuhan 430070, China |
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| Abstract: | The isolation means and properties of morphology and pacemaker current If in Sino-Atrial Node (SAN) cells of rabbits are investigated. In the present study,the SAN trip containing the central nodal region is located by tagging super vena cava with black fine tread as a mark in right atrium of rabbit. The beating pacemaker cell of the SAN is spontaneously isolated by two different enzymatic digestions. Hyperpolarization-activated pacemaker current is recorded by using whole-cell perforated patch technique in... |
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| Keywords: | sino-atrial node cell morphology hyperpolarization-activated pacemaker current |
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