| 怀牛膝毛状根诱导及培养体系的建立 |
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| 引用本文： | 李金亭,张元昊,郭晓双,赵萍萍,杨俊博,张孟凯.怀牛膝毛状根诱导及培养体系的建立[J].河南师范大学学报(自然科学版),2013,41(5):111-114. |
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| 作者姓名： | 李金亭 张元昊 郭晓双 赵萍萍 杨俊博 张孟凯 |
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| 作者单位： | 河南师范大学生命科学学院;河南省高校道地性中药保育及利用工程研究中心,河南新乡453007 |
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| 基金项目： | 国家自然科学基金，河南省重点科技攻关项目，河南省自然科学基金 |
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| 摘 要： | 以牛膝叶片为外植体,研究了不同发根农杆菌、培养基、乙酰丁香酮(AS)对毛状根诱导率及其生长的影响.结果表明,发根农杆菌不同菌种对牛膝叶片转化率有显著影响,3个供试菌种中ATCC15834菌株诱导率最高;在1/2MS无激素固体培养基上毛状根的诱导率及生长状况均优于MS培养基;100μmol·L-1 AS可以有效提高毛状根诱导率.毛状根从叶片形态学下端中脉切口处形成的愈伤组织上产生,能在无外源激素的培养基上自主生长.PCR扩增结果显示发根农杆菌Ri质粒的rolB基因已在牛膝毛状根基因组中插入、整合并得到表达.建立了牛膝毛状根诱导培养体系,为利用毛状根大规模生产药用成分奠定了基础.
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| 关 键 词： | 牛膝 发根农杆菌 毛状根 |
Establishement of Induction and Culture System for Hairy Roots of Achranthes bidentata BL. |
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| LI Jinting
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ZHANG Yuanhao
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GUO Xiaoshuang
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ZHAO Pingping
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YANG Junbo
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ZHANG Mengkai.Establishement of Induction and Culture System for Hairy Roots of Achranthes bidentata BL.[J].Journal of Henan Normal University(Natural Science),2013,41(5):111-114. |
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| Authors: | LI Jinting ZHANG Yuanhao GUO Xiaoshuang ZHAO Pingping YANG Junbo ZHANG Mengkai |
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| Institution: | LI Jinting;ZHANG Yuanhao;GUO Xiaoshuang;ZHAO Pingping;YANG Junbo;ZHANG Mengkai;College of Life Sciences,Henan Normal University;Engineering Technology Research Center of Nursing and Utilization of Genuine Chinese Crude Drugs,University of Henan Province; |
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| Abstract: | The influence of different Agrobacterium rhizogenes strains,medium and acetosyringone on Hairy root induction rate and growth was investigated with leaf explants of A bidentata.The results showed that difference strains have extraordinary influence in leaf transformation,ATCC15834strain led to the highest inducing rate.And the induction and growth rate of hairy roots in 1/2MS medium without auxin was superior to MS medium.100μmol·L-1 Acetosyringone could increase the transformation rate.Hairy roots could be obtained only from the callus formed of basal morphological part petioles of leaf explants of A bidentata.The transformation of hairy roots was confinmed by PCR amplification of rol Bgenes of Ri plasmid fromA.rhizogenes.The initial genetic transformation system of A bidentata was established by ATCC15834.The study can offer the original basis for mass production of medicinal ingredents. |
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| Keywords: | Achranthes bidentata BL Agrobacterium rhizogenes hairy root |
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