| 云南松松针中莽草酸的含量测定 |
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| 引用本文： | 张志琴,刘光明,杨永寿,肖培云.云南松松针中莽草酸的含量测定[J].云南民族大学学报(自然科学版),2012,21(1):10-12. |
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| 作者姓名： | 张志琴 刘光明 杨永寿 肖培云 |
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| 作者单位： | 1. 大理学院药学院,云南大理671000;楚雄医药高等专科学校,云南楚雄675000
2. 大理学院药学院,云南大理,671000 |
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| 基金项目： | 国家自然科学基金(30860365) |
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| 摘 要： | 目的:建立HPLC法测定云南松松针中莽草酸含量的方法.方法:采用Phenomenex Gemi-ni-NX 5μC18110A(4.6mm×250mm)色谱柱,以甲醇∶1%磷酸水溶液(15∶85)为流动相,检测波长215nm,流速1mL/min,柱温为30℃.结果:莽草酸进样量在0.101 2～2.024μg范围内线性关系良好(r=0.999 7),样品的平均回收率为99.76%,RSD为0.89%(n=9).结论:本法操作简便、快速、分离效果好、稳定性高、重现性好,可用于松针中莽草酸的含量测定.
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| 关 键 词： | 高效液相色谱法 云南松 松针 莽草酸 |
Determination of Shikimic Acid in Pine Needles of Pinus yunnanensis Franch |
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| ZHANG Zhi-qin
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LIU Guang-ming
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YANG Yong-shou
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XIAO Pei-yun.Determination of Shikimic Acid in Pine Needles of Pinus yunnanensis Franch[J].Journal of Yunnan Nationalities University:Natural Sciences Edition,2012,21(1):10-12. |
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| Authors: | ZHANG Zhi-qin LIU Guang-ming YANG Yong-shou XIAO Pei-yun |
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| Institution: | 1 (1.College of Pharmacy,Dali University,Dali 671000,China;2 Chuxiong Medical College,Chuxiong 675000,China) |
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| Abstract: | The research aimed to establish a method for the determination of Shikimic acid in Pine needles of Pinus yunnanensis Franch.Phenomenex Gemini-NX 5μ C18 110 A(4.6 mm×250 mm) was used;the mobile phase was methanol∶1% phosphoric acid solution(15∶85);the UV wavelength of shikimic acid was 215nm;the flow rate was 1.0 ml/min;the temperature of column was 30℃.The results showed that Shikimic acid had good linearity over the range of 0.1012-2.024μg(r=0.9997).The average recovery was 99.76%;RSD was 0.89%(n=9).It was concluded that the method is simple,sensitive,accurate and reproducible,which can be applied to determine the content of Shikimic acid in Pine needles of Pinus yunnanensis Franch. |
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| Keywords: | HPLC Pinus yunnanensis Franch Pine needle Shikimic acid |
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