北部湾广西海域海洋真菌多样性及其生物活性研究

本研究从北部湾广西海域采集的海绵、珊瑚、沉积物等样品中分离真菌，旨在挖掘北部湾的海洋真菌资源，筛选出具有潜在抗肿瘤或抗菌活性的菌株。采用稀释涂布法和基于内转录间隔区基因序列(ITS)的系统发育树法分析海洋真菌的多样性信息，并通过滤纸片琼脂扩散法和MTT比色法评价菌株的抗菌、抗肿瘤活性。分离得到45株海洋真菌，隶属17属。其中青霉属(Penicillium)和曲霉属(Aspergillus)为优势种群，分别占总菌株种类的26.7%和24.4%。此外，获得一株潜在的新种菌株Myrothecium gramineum GXIMD01018。活性筛选结果发现菌株Aspergillus japonicus GXIMD01014、Penicillium oxalicum GXIMD01021、Talaromyces purpureogenus GXIMD01024等6株真菌的次级代谢产物对人结直肠癌细胞具有较显著的细胞毒活性，菌株T.purpureogenus GXIMD01024的代谢粗提物具有一定的抗耐甲氧西林金黄色葡萄球菌(Methicillin-Resistant Staphylococc...

Study on the Biodiversity and Biological Activity of Marine Fungi Isolated from Guangxi Beibu Gulf

In this study,fungi were isolated from sponges,corals,sediments and other samples collected from Guangxi Beibu Gulf,aiming to explore the marine fungal resources in Beibu Gulf and screen out strains with potential anti-tumor or antibacterial activity.The diversity information of marine fungi was analyzed by dilution coating method and phylogenetic tree method based on Internal Transcribed Spacer (ITS) gene sequence.The antibacterial and antitumor activities of the strains were evaluated by filter paper agar diffusion method and MTT colorimetry.45 strains of marine fungi were isolated,belonging to 17 genera.Among them,Penicillium and Aspergillus were the dominant species,accounting for 26.7% and 24.4% of the total strains,respectively.In addition,a potential new strain Myrothecium gramineum GXIMD01018 was obtained.The results of activity screening showed that the secondary metabolites of Aspergillus japonicus GXIMD01014,Penicillium oxalicum GXIMD01021 and Talaromyces purpureogenus GXIMD01024 had significant cytotoxic activity against human colorectal cancer cells.The crude extract of strain T.purpureogenus GXIMD01024 had certain activity against Methicillin-Resistant Staphylococcus aureus (MRSA).This study provides a scientific basis for the discovery of new anti-tumor and anti-bacterial lead compounds from marine sources.