| 核桃DNA的提取及RAPD体系的优化 |
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| 引用本文： | 张虎平,牛建新,马兵钢,虎海防,王国安. 核桃DNA的提取及RAPD体系的优化[J]. 石河子大学学报(自然科学版), 2003, 7(4): 267-270 |
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| 作者姓名： | 张虎平 牛建新 马兵钢 虎海防 王国安 |
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| 作者单位： | 石河子大学农学院园艺系,新疆,石河子,832003;新疆林业科学院,新疆,乌鲁木齐,832000 |
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| 基金项目： | 新疆自然科学基金资助项目(211003) |
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| 摘 要： | 采用CTAB法和改进的CTAB法,从叶片中提取核桃基因组DNA,比较其分离效果,结果表明：改进的CTAB法可有效去除细胞内多糖和多酚等杂质对模板DNA的污染,并以此为模板进行RAPD反应,对RAPD反应程序中的一些重要参数进行摸索和优化试验,建立适合于核桃的RAPD-PCR反应体系,以进行该树种的分子标记研究。
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| 关 键 词： | 核桃 DNA提取 RAPD |
| 文章编号： | 1007-7383(2003)04-0267-04 |
| 修稿时间： | 2003-04-21 |
Method of DNA Extraction and RAPD Reaction System in Walnut |
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| ZHANG Hu-ping,NIU Jian-xin,MA Bing-gang,HU Hai-fang,WANG Guo-an. Method of DNA Extraction and RAPD Reaction System in Walnut[J]. Journal of Shihezi University(Natural Science), 2003, 7(4): 267-270 |
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| Authors: | ZHANG Hu-ping NIU Jian-xin MA Bing-gang HU Hai-fang WANG Guo-an |
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| Abstract: | Two methods(CTAB and reformed CTAB)were adopted to isolate genomic DNA of Walnut( Juglans rejia L) . The results show that the reformed CTAB is more effective to remove most of polysaccharides in the cytoplasmic and to inhibit the formation of oxidized polyphenolic compounds. Based on the genomic DNA extracted from Walnut, essential factors affecting the result of RAPD assay were tested and compared. An optimal reaction system suitable for the assay and usage of RAPD in Walnut was established. |
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| Keywords: | Walnut DNA extract RAPD |
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