| 壬基酚或双酚A对原代培养鲫肝细胞毒性的影响 |
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| 引用本文： | 郭彤,张高峰,朱宝长.壬基酚或双酚A对原代培养鲫肝细胞毒性的影响[J].首都师范大学学报(自然科学版),2009,30(2):35-40. |
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| 作者姓名： | 郭彤 张高峰 朱宝长 |
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| 作者单位： | 1. 首都师范大学生命科学学院,北京,100048
2. 同济大学生命科学与技术学院,上海,200092 |
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| 基金项目： | 上海市教委发展基金，上海市重点学科建设项目 |
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| 摘 要： | 以纯化的鲫（Carassius auratus）肝细胞为试验对象,研究不同浓度壬基酚（NP）或双酚A（BPA）对肝细胞增殖、乳酸脱氢酶（LDH）释放,丙二醛（MDA）含量,谷胱甘肽-s转移酶（GST）,7-乙氧异吩噁唑酮-O-脱乙基酶（EROD）活性的影响.结果表明,鲫肝细胞暴露于不同浓度的NP或BPA 24 h后,随着NP、BPA浓度的升高,细胞增殖率明显降低（P〈0.05）;肝细胞LDH的渗出量、MDA、GST及EROD活性均显著增加（P〈0.05）.表明,NP或BPA对鲫肝细胞增具有一定的毒性作用.而且BPA对肝细胞的毒害作用要弱于NP（P〈0.05）.
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| 关 键 词： | 壬基酚 双酚A 肝细胞 乳酸脱氢酶 丙二醛 谷胱甘肽-s转移酶 7-乙氧异吩噁唑酮-O-脱乙基酶 |
Toxic Effection of 4-nonylphenol or Bisphenol A on Primary Culture Hepatocytes of Carassium Auratus |
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| Guo Tong,Zhang Gaofeng,Zhu Baochang.Toxic Effection of 4-nonylphenol or Bisphenol A on Primary Culture Hepatocytes of Carassium Auratus[J].Journal of Capital Normal University(Natural Science Edition),2009,30(2):35-40. |
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| Authors: | Guo Tong Zhang Gaofeng Zhu Baochang |
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| Institution: | 1;1 College of life Science;Capital Normal University;Beijing 100048;2 College of life Science and Technology;Tongji University;Shanghai 200092 |
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| Abstract: | In this study,purified hepatocytes of Carassius auratus were used as a experimental objection.In order to study the toxic effect of different concentration 4-Nonylphenol or Bisphenol A on hepatocytes of Carassius auratus,cell proliferation,lactate dehydrogenase(LDH) release,malonaldehyde(MDA),glutathione stransferase(GST) and 7-Ethoxyresorufin-O-deethylase(EROD) activities were measured.The results showed that proliferation of hepatocytes was decreased(P<0.05) with the concention of NP or BPA increasing.Res... |
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| Keywords: | 4-Nonylphenol Bisphenol A Hepatocytes Lactate dehydrogenase Malonaldehyde Glutathione stransferase 7-Ethoxyresorufin-O-deethylase |
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