Ochrobactrum lupini TP-D1降解百菌清产物的研究

苍白杆菌(Ochrobactrum lupini)TP-D1可降解百菌清。采用硅胶薄层层析(TLC)法对TP-D1降解百菌清的产物进行分离。TP-D1接种于含50mg/L百菌清的培养液中，30℃，180r/min培养4天，培养液点于GF254层析板上，以正己烷、异丙醇、甲醇体积比5∶3∶2为展开剂进行层析分离，于254nm紫外光下收集分离斑，甲醇溶解、自然浓缩后，利用高效液相色谱一质谱联用技术(HPLC-MS)分析鉴定，获得两种百菌清微生物降解产物，分别为1,3-二酰胺-四氯苯和4-羟基-三氯苯二腈(TPN-OH)，推测TP-D1通过氰基的水合作用和羟基化作用两种途径降解百菌清。

Detection of the biodegradation products of Chlorothalonil by Ochrobactrum lupini TP-D1

Chlorothalonil can be degraded by ochrobactrum lupini TP-D1. The metabolic substances of Chlorothalonil cultured with TP D1 at 30?℃ for 4 days were separated by thin layer chromatography (TLC) using 5∶3∶2 hexane-Isopropyl alcohol-methanol mixture developing solvent (volumn fraction). The developed strip of the metabolites was collected under the ultraviolet radiation (254nm), dissolved in methanol, and naturally concentrated. Two biodegradation products were determined by the analysis of LC-MS, one is 1,3-dicarbamoyl 2,4,5,6-tetrachlo-robenzene and another is 4-hydroxy-2,5,6-trichlorois-ophthalonitril (TPN-OH). It was supposed that Chlorothalonil was degraded by TP-D1 through the direct hydroxylation of Chlorothalonil and the hydration of its cyano groups.