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| 壳聚糖微球亲和层析提取抑酶肽的研究 | |
| 引用本文： | 范继业,张 静,高冬婷,王 刚,刘 然.壳聚糖微球亲和层析提取抑酶肽的研究[J].河北科技大学学报,2009,30(2):92-94,98. |
| 作者姓名： | 范继业 张 静 高冬婷 王 刚 刘 然 |
| 作者单位： | 1. 河北化工医药职业技术学院制药工程系,河北石家庄,050026 2. 河北化工医药职业技术学院制药工程系,河北石家庄,050026;河北科技大学化学与制药工程学院,河北石家庄,050018 3. 石家庄药集团中诺药业有限公司,河北石家庄,050051 |
| 摘 要： | 建立了一种高效、实用的抑酶肽制备技术。采用牛肺为原料,利用改性壳聚糖载体,共价偶联胰蛋白酶,亲和层析牛肺提取液,2步制得高比活抑酶肽。抑酶肽抑制比活力为71428 BAEE/mg,酶活性回收率为73.5%。该方法质量稳定,成本较低,吸附剂机械强度高,抗污染能力较强,可反复使用,适宜应用。 |
| 关 键 词： | 牛肺 亲和层析 抑酶肽 壳聚糖 |
| 收稿时间： | 2009/2/19 0:00:00 |
| 修稿时间： | 2009/4/1 0:00:00 |
Preparation of high purity aprotinin by globate cross-linked chitosan |
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| FAN Ji-ye,ZHANG Jing,GAO Dong-ting,WANG Gang and LIU Ran.Preparation of high purity aprotinin by globate cross-linked chitosan[J].Journal of Hebei University of Science and Technology,2009,30(2):92-94,98. | |
| Authors: | FAN Ji-ye ZHANG Jing GAO Dong-ting WANG Gang and LIU Ran |
| Institution: | 1.Department of Pharmaceutical Engineering;Hebei Chemical and Pharmaceutical Vocational Technology College;Shijiazhuang Hebei 050026;China;2.College of Chemical and Pharmaceutical Engineering;Hebei University of Science and Technology;Shijiazhuang Hebei 050018;3.Zhongnuo Pharmaceutical Company Limited;CSPC;Shijiazhuang Hebei 050051;China |
| Abstract: | The research aimed to establish a kind of effective and practical method to produce aprotinin.The trypsin was covalently linked with chemical modified chitosan and used to purify aprotinin from the extract of cattle lungs by affinity chromatography.The result shows that the specific activity of immobilized trypsin on chitosan is 71 428 BAEE/mg,and the activity recovery of trypsin is 73.5%.It is accepted as a stable and low-cost method and suitable for purifying aprotinin with high activity in industrial man... |
| Keywords: | cattle lung affinity chromatography aprotinin chitosan |
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