云南高海拔牧场牦牛捕食线虫真菌生防高效菌株的筛选

目的:为筛选云南高海拔牧场牦牛高效力的寄生线虫生防真菌菌株。方法:对云南大理花甸坝牧场,中甸打日坝牧场及白马雪山牧场采集的少孢节丛孢(A.oligospora)、弯孢节丛孢(A.musiformis)、椭圆隔指孢(D.ellipsospora)和长孢隔指孢(D.leptospora)采用盐酸胃蛋白酶处理筛选耐受菌株,并进一步比较了耐受菌株的捕食活力。结果:12株捕食线虫真菌中,有6株通过盐酸胃蛋白酶处理,通过率为50%,其中大理花甸坝和中甸打日坝的A.oligospora及白马雪山牧场的D.ellipsospora显示出较强的捕食活力。结论:本研究初步筛选出3株生防高效菌株,为牦牛及其它动物线虫病的生物防治积累了捕食线虫真菌菌种资源。

Screening of High-performance Biocontrol Strains of Nematode-trapping Fungi from Yak Dung in Plateau Pastures of Yunnan Province

Objective： To screen high efficiency biocontrol strains of nematode-trapping fungi isolated from yak dung in plateau pastures of Yunnan Province. Methods：Twelve nematode-trapping fungi of A. oligospora, A. musiformis, D. ellipsospora, D. leptospora isolated from Dali Huadianba pasture, Zhongdian Dariba pasture, Baima Snow Mountain pasture were tested in pepsin-hydrochloric acid and the nematode-trapping activity was determined subsequently. Results：Half of isolates survived in pepsin-hydrochloric acid treatment and three isolates A. oligospora from Huadianba pasture and Dariba pasture and D. ellipsospora from Baima Snow Mountain pasture displayed high nematode-trapping activity under laboratory conditions. Conclusion： Three high efficiency biocontrol nematode-trapping fungi were obtained and this research accumulated biocontrol strain resources in the biological control of nematode disease of yak and other animals.