| 人类锌指蛋白新基因ZNF641的克隆及表达 |
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| 引用本文： | 齐兴柱,朱伟,吴秀山. 人类锌指蛋白新基因ZNF641的克隆及表达[J]. 海南大学学报(自然科学版), 2008, 26(1): 54-62 |
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| 作者姓名： | 齐兴柱 朱伟 吴秀山 |
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| 作者单位： | 海南大学,海洋学院,海南,海口,570228;海南大学,海洋学院,海南,海口,570228;海南大学,海洋学院,海南,海口,570228 |
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| 基金项目： | 国家自然科学基金 , 湖南省科研项目 , 教育部湖南省教育厅基金 , 海南大学校科研和教改项目 |
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| 摘 要： | 从人类心脏cDNA文库中分离并鉴定了一个新的人类锌指基因ZNF641，该基因的cDNA序列全长4．9kb，编码一个含438个氨基酸的蛋白质，从进化上看，该蛋白质在从小鼠到人的脊椎动物中都高度保守．Northern Blot分析显示：ZNF641在人类的多数组织中都有表达，尤其是在骨骼肌中有较高水平的表达．而亚细胞定位则显示ZNF641在细胞核及细胞质中均有定位．
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| 关 键 词： | ZNF641 KRAB框 亚细胞定位 表达 |
| 文章编号： | 1004-1729(2008)01-0054-09 |
| 修稿时间： | 2007-04-26 |
Cloning and Expression Analysis of a Novel Human Zinc-finger Protein ZNF641 |
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| QI Xing-zhu,ZHU Wei,WU Xiu-shan. Cloning and Expression Analysis of a Novel Human Zinc-finger Protein ZNF641[J]. Natural Science Journal of Hainan University, 2008, 26(1): 54-62 |
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| Authors: | QI Xing-zhu ZHU Wei WU Xiu-shan |
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| Affiliation: | (College of Ocean, Hainan University, Haikou 570228, China) |
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| Abstract: | This paper presents the identification and characterization of a novel human zinc finger protein, and ZNF641 from human heart cDNA library. The cDNA of ZNF641 is 4.9 kb, encoding 438 amino acids in the nucleus. The protein is highly conserved in evolution across different vertebrate species from mouse to human. Northern blot analysis indicates that ZNF641 is expressed in most of the examined human tissues, with a high level in skeletal muscle. Sub-cellular localization shows a nuclear and cytoplasm fluorescence pattern. |
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| Keywords: | ZNF641 KRAB motif Sub-cellular localization Expression |
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