| 海带多糖中蛋白质去除方法的对比研究 |
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| 引用本文： | 余华.海带多糖中蛋白质去除方法的对比研究[J].成都大学学报(自然科学版),2005,24(4):265-268. |
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| 作者姓名： | 余华 |
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| 作者单位： | 成都大学,生物工程系,成都,610106 |
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| 基金项目： | 四川省成都市科技局资助项目 |
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| 摘 要： | 采用4种方法对海带多糖进行了脱蛋白研究,并比较了脱蛋白效果与多糖损失之间的关系.结果表明:这四种方法脱蛋白效果优劣顺序依次为蛋白酶解法、三氯乙酸沉淀法、鞣酸沉淀法和Sevag法.脱蛋白过程中使蛋白质沉淀的机理可能是造成多糖损失的主要原因,蛋白酶解法脱蛋白效果最好且多糖得率最高.
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| 关 键 词： | 海带多糖 脱蛋白 多糖损失 对比研究 |
| 文章编号： | 1004-5422（2005）04-0265-04 |
| 收稿时间： | 2005-09-29 |
| 修稿时间： | 2005-09-29 |
Study on in Four Methods in Comparison Removing of Protein from Polysaccharide of Laminaria Japonica Aresch |
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| YU Hua.Study on in Four Methods in Comparison Removing of Protein from Polysaccharide of Laminaria Japonica Aresch[J].Journal of Chengdu University (Natural Science),2005,24(4):265-268. |
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| Authors: | YU Hua |
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| Institution: | Department of Bioengineering, Chengdu University, Chengdu 610106, China |
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| Abstract: | In this paper,four kinds of methods of removing protein from Laminaria japonica Aresch polysaccharide were studied.And the relationship between the effect of removing protein and the loss of Laminaria japonica Aresch polysaccharide was compared.The results show that the order of these four kinds of methods of removing protein is as follows:protease,trichloroaceticacid,tannic acid and Sevag.The mechanism making the protein precipitate might be the main reason that caused polysaccharide losses while removing protein,with the protease is the best in taking off the protein and with the highest rate of Laminaria japonica Aresch polysaccharide. |
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| Keywords: | Laminaria japonica Aresch polysaccharide removing protein polysaccharide loss comparion |
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