亚临界水法制备克拉霉素纳米颗粒

采用亚临界水法制备了克拉霉素纳米颗粒，通过调节亚临界水温度、反溶剂（普通去离子水）温度、亚临界水/反溶剂体积比、表面活性剂浓度等因素，得到形貌均匀的克拉霉素纳米颗粒。优化后的工艺条件为：亚临界水、反溶剂的温度分别为140℃，0℃，亚临界水/反溶剂体积比为1∶3，搅拌时间25min，表面活性剂α-乳糖质量分数为0.4%。在上述条件下制备得到的颗粒的平均粒径为65nm左右，原料药和样品的红外光谱图和X射线衍射对比结果显示其化学结构没有发生变化。溶出结果表明，亚临界水法制备的克拉霉素纳米颗粒溶出度有了明显的提高。

Preparation of clarithromycin nanoparticles by a subcritical water method

Nanoparticles of clarithromycin have been obtained by using subcritical water (SBCW) technology. Uniform clarithromycin nanoparticles were obtained by adjusting the temperature of subcritical water, anti-solvent (deionized water) temperature, subcritical water/anti-solvent volume ratio and surfactant concentration. The optimized technological conditions were found to be as follows: the temperatures of subcritical water and anti-solvent were 140℃,and 0℃ respectively, the volume ratio was 1∶3, the stirring time was 25min, the mass fraction of α-lactose was 0.4%, and the average particle diameter of the particles was 65nm. Infrared spectra and XRD of the starting materials and final samples showed that no chemical reaction occured during the experiments. In dissolution rate tests, the dissolution rate of clarithromycin nanoparticles prepared using subcritical water was shown to be significantly improved. 