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| 两步法活化微晶纤维素及在固定木瓜蛋白酶中的应用 | |
| 引用本文： | 郭刚军,马林,谢文林,陈国雄,古练权. 两步法活化微晶纤维素及在固定木瓜蛋白酶中的应用[J]. 中山大学学报(自然科学版), 2001, 40(3): 45-47 |
| 作者姓名： | 郭刚军 马林 谢文林 陈国雄 古练权 |
| 作者单位： | 中山大学化学与化学工程学院, |
| 基金项目： | 国家自然科学基金资助项目(20074042和20032020) |
| 摘 要： | 微晶纤维素经过两步活化，可在温和条件下实现木瓜蛋白酶固定化，用对甲基苯碘酰氯活化微晶纤维素后，与乙二胺反向载体引入有机胺基因，再用戊二醒处理所得的二次活化载体可在温和条件固定木瓜蛋白酶，所得固定化酶活力为103U／g载体，与溶液酶比较，热稳定性有明显提高。 |
| 关 键 词： | 微晶纤维素 木瓜蛋白酶 固定化 两步法活化 |
| 文章编号： | 0529-6579(2001)03-0045-03 |
| 修稿时间： | 2000-09-04 |
Activation of Microcrystalline Cellulose by Two-step Method and Its Use in Immobilization of Papain |
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| GUO Gang-jun,MA Lin,XIE Wen-lin,CHEN Guo-xiong,GU Lian-quan. Activation of Microcrystalline Cellulose by Two-step Method and Its Use in Immobilization of Papain[J]. Acta Scientiarum Naturalium Universitatis Sunyatseni, 2001, 40(3): 45-47 | |
| Authors: | GUO Gang-jun MA Lin XIE Wen-lin CHEN Guo-xiong GU Lian-quan |
| Abstract: | Immobilized papain was prepared using microcrystalline cellulos as support which was activated by two_step method.The microcrystalline cellulose was first activated with %p%_toluenesulfonyl chloride, followed by coupling with ethyl diamine.After treated with glutaraldehyde, papain was immobilized to the support.Immobilized papain was obtained with activity 103 U/g support and high stability. |
| Keywords: | immobilization microcrystalline cellulos papain |
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