| 纳米金增强DNA修饰金电极电化学性能的研究 |
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| 引用本文： | 周路,袁若,柴雅琴,傅英姿,王秀玲,黄小清,李群芳.纳米金增强DNA修饰金电极电化学性能的研究[J].西南师范大学学报(自然科学版),2004,29(5):789-794. |
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| 作者姓名： | 周路 袁若 柴雅琴 傅英姿 王秀玲 黄小清 李群芳 |
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| 作者单位： | 西南师范大学,化学化工学院,重庆市现代分析化学重点实验室,重庆,400015 |
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| 基金项目： | 国家自然科学基金资助项目(29705001)，教育部优秀青年教师资助计划(教人司2002-40)，重庆市自然科学基金资助项目(2002-7477). |
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| 摘 要： | 采用小牛胸腺DNA修饰金电极并用纳米金增强了DNA在电极上的吸附量.Co(bpy)3 3被用作电子媒介体来表征通过恒电位法吸附在电极表面的DNA的变化过程,并通过循环伏安法测定Co(bpy)3 3在电极上的富集量;同时,考察了DNA修饰金电极在不同pH值及不同温度下的稳定性,发现纳米金使DNA修饰电极稳定性增加.
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| 关 键 词： | 巯基乙胺 纳米金 小牛胸腺DNA 电化学性能 |
Colloid Au-Enhanced DNA Immobilization for the Electrochemical Characterization of Gold Electrodes (Part Ⅱ ) |
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| Abstract.Colloid Au-Enhanced DNA Immobilization for the Electrochemical Characterization of Gold Electrodes (Part Ⅱ )[J].Journal of Southwest China Normal University(Natural Science),2004,29(5):789-794. |
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| Authors: | Abstract |
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| Abstract: | We use calf-thymus DNA(CT DNA) to modify the gold electrode and colloid Au to enhance the DNA immobilization amount on the electrode. The DNA modified electrode was electrochemically characterized with Co(bpy)~(3 )_3, a electroactive DNA-binding complex, as an indicator. The amount of Co(bpy)~(3 )_3 enriched by the layers of DNA LSV(linear sweap voltammetry) adsorbed at the electrode was estimated from the peak charge of 5 mmol/L Co(bpy)~(3 )_3 reduction at the electrode obtained by cycle voltmmetry(CV). The stability of the DNA-modified electrode was investigated and the results obtained exhibit that the colloidal Au make the DNA-modified gold electrode stable to alkali, acid and hot water compared to the bare gold electrode. |
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| Keywords: | cysteamine colloidal Au calf-thymus DNA electrochemical characterization |
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