| HMC毒素对同核异质玉米叶肉细胞原生质体的影响 |
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| 引用本文： | 陈颖,杨虹,霍晨敏,王立安.HMC毒素对同核异质玉米叶肉细胞原生质体的影响[J].河北师范大学学报(自然科学版),2007,31(5):662-665. |
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| 作者姓名： | 陈颖 杨虹 霍晨敏 王立安 |
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| 作者单位： | 1. 河北经贸大学,生物科学与工程学院,河北,石家庄,050061
2. 河北师范大学,生命科学学院,河北,石家庄,050016 |
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| 摘 要： | 制备并纯化了玉米小斑病菌C小种致病毒素(HMC),利用根冠细胞进行毒素活性检测,结果表明其对玉米C型不育细胞质具有专化活性.同时提取了同核异质玉米C103叶肉细胞原生质体,并用纯化后不同质量浓度的HMC毒素对同核异质C103叶肉细胞原生质体进行处理,光学显微镜下观察并统计HMC毒素对同核异质玉米叶肉细胞原生质体的影响,从而对玉米小斑病菌C小种的致病性进行研究.研究结果表明,HMC毒素可以导致C103玉米C型不育细胞质叶肉细胞原生质体质膜破裂,细胞内容物泄漏.
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| 关 键 词： | 玉米 HMC 叶肉细胞 原生质体 |
| 文章编号： | 1000-5854（2007）05-0662-04 |
| 修稿时间： | 2007-03-092007-04-20 |
Effects of HMC Toxin on Protoplasts of Charrua Male-sterility Maize |
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| CHEN Ying,YANG Hong,HUO Chen-min,WANG Li-an.Effects of HMC Toxin on Protoplasts of Charrua Male-sterility Maize[J].Journal of Hebei Normal University,2007,31(5):662-665. |
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| Authors: | CHEN Ying YANG Hong HUO Chen-min WANG Li-an |
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| Institution: | 1. College of Biology Science and Engineering,Hebei University of Economic and Business,Hebei Shijiazhuang 050061 ,China; 2. College of Life Scienee,Hebei Normal University,Hebei Shijiazhuang 050016,China |
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| Abstract: | A toxin preparation from Bipolaris(Helminthosporium) maydis Race C(HMC-toxin) with apparently biological activities was special active against root cap cells from Charrua male-sterile(C) cytoplasm corn.Protoplasts from mesophyll cells of Charrua male-sterile(C) cytoplasm corn were extracted and treated with specific toxin so as to study the mechanism of Bipolaris(Helminthosporium) maydis Race C.The microscocpe observation showed that the protoplasts were destroyed following the cytoplasts leaking out.The statistics indicated that HMC toxin was specific to destroy the protoplasts of the Charrua male-sterile(C) cytoplasm. |
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| Keywords: | corn HMC mesophyll cells protoplasts |
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