| 玉米PDE191基因全长cDNA的克隆及其生物信息学分析 |
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| 引用本文： | 熊伟,刘久东,杨飞,孙美涛,张海洋.玉米PDE191基因全长cDNA的克隆及其生物信息学分析[J].井冈山大学学报（自然科学版）,2016(5):36-42,57. |
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| 作者姓名： | 熊伟 刘久东 杨飞 孙美涛 张海洋 |
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| 作者单位： | 大理大学基础医学院, 云南, 大理 671000;云南省昆虫生物医药研发重点实验室, 云南, 大理 671000,江苏省仪征市农业委员会, 江苏, 仪征 211400,云南农业大学高原特色农业产业研究院, 云南, 昆明 650100,大理大学基础医学院, 云南, 大理 671000,大理大学基础医学院, 云南, 大理 671000 |
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| 基金项目： | 云南省教育厅科学研究重点项目（2014Z126）；云南省昆虫生物医药研发重点实验室开放课题（201409）；大理学院博士科研启动基金项目（BSKY2012018）；大理学院应用开发研究基金项目（201405）；云南省大学生创新创业训练计划项目（201408） |
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| 摘 要： | 为研究玉米白化相关基因PDE191序列信息和基因功能,以玉米叶片总RNA逆转录后的c DNA为模板,采用RT-PCR和RACE技术克隆出玉米PDE191基因全长c DNA。DNA测序及生物信息学分析表明,玉米PDE191基因c DNA全长1229 bp,编码333个氨基酸的蛋白质,亚细胞定位于线粒体和叶绿体。软件预测PDE191蛋白分子量为37.79 k D,理论等电点为9.12,该蛋白二级结构主要构成元件为?螺旋和无规则卷曲,包含7个MTERF结构域。三级结构预测显示该蛋白为球状结构,预测结果与二级结构预测的结果相符。对该蛋白进行多重序列比对和聚类分析显示,PDE191属于植物MTERF家族,在不同植物中存在其同源蛋白质,其中与拟南芥PDE191氨基酸序列的相似性高达99%。本研究为进一步研究玉米PDE191基因的功能奠定了基础。
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| 关 键 词： | 玉米 PDE191基因 全长cDNA 生物信息学分析 |
| 收稿时间： | 4/6/2016 12:00:00 AM |
| 修稿时间： | 2016/6/16 0:00:00 |
CLONING AND BIOINFORMATICS ANALYSIS OF FULL LENGTH cDNA OF PDE191 GENE IN ZEA MAYS |
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| XIONG Wei,LIU Jiu-dong,YANG Fei,SUN Mei-tao and ZHANG Hai-yang.CLONING AND BIOINFORMATICS ANALYSIS OF FULL LENGTH cDNA OF PDE191 GENE IN ZEA MAYS[J].Journal of Jinggangshan University(Natural Sciences Edition),2016(5):36-42,57. |
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| Authors: | XIONG Wei LIU Jiu-dong YANG Fei SUN Mei-tao and ZHANG Hai-yang |
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| Institution: | Basic School of Medicine, Dali University, Dali, Yunnan 671000, China;Yunnan Provincial Key Laboratory of Entomological Biopharmaceutical R & D, Dali, Yunnan 671000, China,Yizheng Agricultural Committee, Yizheng, Jiangsu 211400, China,Plateau Characteristic Agriculture Research Institute of Yunnan Agricultural University, Kunming, Yunnan 650100, China,Basic School of Medicine, Dali University, Dali, Yunnan 671000, China and Basic School of Medicine, Dali University, Dali, Yunnan 671000, China |
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| Abstract: | To research bioinformation and biological function of the albinism-related PDE191,RT-PCR and RACE technique were used to clone the full length cDNA of PDE191 in Zea mays,and many bioinformatics methods were used to analyze its structure and function.The results showed that,full length cDNA of Z.mays PDE191 was 1229bp,encoded the hydrophilic protein consist of 333 amino acids and localized in mitochondria and chloroplast.The calculated molecular mass of Z.mays PDE191 was 37.79 kD.The theoretical isoelectric point was 9.12.Main composition of the PDE191 protein secondary structure was a-helix and random coil,containing seven MTERF motifs.The multiple alignment and clustering analysis showed that,Z.mays PDE191 was a protein belong to plant MTERF family,and had high similarity to Arabidopsis thaliana PDE191.The cloning of Z.mays PDE191 and bioinformatics analysis results would provide foundation for the further study of its function. |
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| Keywords: | Zea mays pigment defective 191 gene full length cDNA bioinformatics analysis |
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