Activation of proteinkinase ERK mediates induction of macrophage MMP-12 by OxLDL |
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Authors: | He?Chun-yan Email author" target="_blank">Zhou?XinEmail author Li?Xiao-ming Yu?Hong Hong?Jia-ling |
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Institution: | (1) Zhongnan Hospital, School of Medicine, Wuhan University, 430071 Wuhan, Hubei, China;(2) Department of Biochemistry and Molecular Biology, School of Medicine, Wuhan University, 430071 Wuhan, Hubei, China |
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Abstract: | The present study was undertaken to investigate the effect of oxidized low density lipoprotein (oxLDL) on the expression of
macrophage matrix metalloproteinase-12 (MMP-12), and the possible mechanisms. Activation of extracellular signal-regulated
kinase 1/2 (ERK1/2) was detected by Western blot Analysis. Enzymatic activity of MMP-12 was determined by β-casein zymography.
RT-PCR analysis was used to measure the mRNA expression level of MMP-12. OxLDL-stimulated macrophages produced increased casein-degrading
activities and oxLDL also significantly increased the mRNA level of MMP-12 in a dose-dependent manner. OxLDL stimulated the
phosphorylation of ERK1/2 in macrophages. The use of the specific inhibitor indicated that the ERK1/2 signaling pathway was
required for the induction of MMP-12. These data demonstrated that oxLDL induced MMP-12 expression in macrophages through
an ERK1/2-dependent pathway.
Foundation item: Supported by the Natural Science Foundation of Hubei Province (99J138)
Biography: He Chun-yan (1970-), female, Ph. D candidate, research direction: biochemistry diagnosis of atherosclerosis. |
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Keywords: | oxidized low density lipoproteins macrophage matrix metalloproteinases |
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