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虎杖内生真菌抗菌活性研究及菌株初步鉴定
引用本文:彭浩,陈文强,邓百万,兰阿峰.虎杖内生真菌抗菌活性研究及菌株初步鉴定[J].陕西理工学院学报(自然科学版),2014(4):61-65.
作者姓名:彭浩  陈文强  邓百万  兰阿峰
作者单位:陕西理工学院生物科学与工程学院,陕西省食药用菌工程技术研究中心,陕西汉中723000
基金项目:基金项目:陕西省教育厅2014年科学研究计划项目
摘    要:利用滤纸片扩散法及形态学常规分类法对30株虎杖内生真菌发酵液进行抑菌活性试验和初步鉴定。结果表明,15株内生真菌对金黄色葡萄球菌(Staphylococcus aureus)具有抑菌活性,12株内生真菌对枯草芽孢杆菌(Bacillus subtilis)具有抑菌活性,7株内生真菌对大肠埃希氏菌(Escherichia coli)具有抑菌活性,分别占菌株总数的50%,40%和23%。根据菌落形态及菌丝结构对其鉴定,具有抑菌活性的菌株分属于曲霉属(Aspergillus)、青霉属(Penicillium)、枝孢霉属(Cladosporium)、镰刀菌属(Fusarium)及无孢菌类(Agonomycetales)真菌。

关 键 词:虎杖  内生真菌  抗菌活性  鉴定

Study on antibacterial activity and preliminary identification of endophytic fungi from Polygonum cuspidatum
PENG Hao,CHEN Wen-qiang,DENG Bai-wan,LAN A-feng.Study on antibacterial activity and preliminary identification of endophytic fungi from Polygonum cuspidatum[J].Journal of Shananxi University of Technology:Natural Science Edition,2014(4):61-65.
Authors:PENG Hao  CHEN Wen-qiang  DENG Bai-wan  LAN A-feng
Institution:( School of Biological Science and Engineering, Shaanxi University of Technology, Shaanxi Engineering Research Center of Edible and Medical Fungi, Hanzhong 723000, China)
Abstract:Antibacterial activities of 30 strains of endophytic fungi from Polygonum cuspidatum were studied by filter paper method. The results showed that Staphylococcus aureus was inhibited by 15 strains,Bacillus subtilis inhibited by 12 strains and Escherichia coli inhibited by 7 strains,rspectively accounting for 50% ,40% and 23% of the total. According to the morphological and structural characteristics,the strains with antibacterial activity belong to the genus Aspergillus sp. ,Penicillium sp. ,Cladosporium sp. ,Fusarium sp. and Agonomycetales sp.
Keywords:Polygonum cuspidatum  endophytic fungi  antibacterial activities  identification
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