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泗洪大枣组培繁殖技术
引用本文:蒋泽平,梁珍海,刘根林,李玉巧.泗洪大枣组培繁殖技术[J].南京林业大学学报(自然科学版),2004,28(4):97-100.
作者姓名:蒋泽平  梁珍海  刘根林  李玉巧
作者单位:江苏省林业科学研究院,江苏,南京,211153
基金项目:“西南林区天然林保育技术研究与示范”子课题
摘    要:泗洪大枣(Zizyphus jujuba Mill.var.sihongensis)组培繁殖技术研究结果表明,促进泗洪大枣试管苗茎、叶营养生长效果最佳的培养基配方为改良WPM+1.0mg/LBA+0.005mg/LTDZ+0.2mg/LIBA+3.0%S,40d生长高度达3.8cm;促进增殖最佳培养基配方为改良WPM+1.5mg/LBA+0.005mg/LTDZ+0.15mg/LIBA+3.0%S,35d增殖4.5倍;促使试管苗产生优质根系的最佳培养基配方为1/2WPM+0.5mg/I.IBA+0.2mg/LNAA+2.0%S,试管苗生根率达90%以上,且发根粗而短,生理活性旺盛;生根试管苗在V砻糠灰:V黄心土=3:2混合的基质中移栽,成活率最高达85.0%。

关 键 词:泗洪大枣  植物生长调节剂  试管苗
文章编号:1000-2006(2004)04-0097-04

The Technique of the Tissue Culture of Zizyphus jujuba Mill.Var.Sihongensis
JIANG Ze-ping,LIANG Zhen-hai,LIU Gen-lin,LI Yu-qiao.The Technique of the Tissue Culture of Zizyphus jujuba Mill.Var.Sihongensis[J].Journal of Nanjing Forestry University(Natural Sciences ),2004,28(4):97-100.
Authors:JIANG Ze-ping  LIANG Zhen-hai  LIU Gen-lin  LI Yu-qiao
Abstract:The technique of the tissue culture of Zizyphus jujuba Mill.var.sihongensis showed that modified WPM+1.0 mg/L BA+0.005 mg/L TDZ+0.2 mg/L IBA+3.0% S optimized the tube-shoot growth,and in this medium the height growth went up to 3.8 cm for 40 days.Modified WPM+1.5 mg/L BA+0.005 mg/L TDZ+0.15 mg/L IBA+3.0% S optimized the tube-shoot multiplication,and in this medium the multiplication time went up to (4.5) for 35 days.1/2 WPM+0.5 mg/L IBA+0.2 mg/L NAA+2.0% S optimized the tube-shoot rooting percentage reached 90%.And the plantlets transplanted in the substract containing three parts of chaff ash and two parts of garden earth acclimatized well and the survival rate got up to 85.0%.
Keywords:Zizyphus jujuba Mill  var  sihongensis  Plant growth regulator  Tube-shoot
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