| 根癌农杆菌介导的GFP在洋葱表皮细胞定位研究 |
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| 引用本文： | 刘肖飞,梁卫红.根癌农杆菌介导的GFP在洋葱表皮细胞定位研究[J].河南师范大学学报(自然科学版),2009,37(1). |
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| 作者姓名： | 刘肖飞 梁卫红 |
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| 作者单位： | 河南师范大学,生命科学学院,河南,新乡,453007 |
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| 基金项目： | 河南省高等学校青年骨干教师资助计划，河南省科技攻关项目 |
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| 摘 要： | 采用根癌农杆菌介导的方法,以受控于CaMV35S启动子的携带有GFP报告基因的双元植物表达载体pCAMBIA1300-35S-GFP转化洋葱表皮细胞.荧光显微镜下观察结果显示,GFP基因在经浸染和共培养后的洋葱表皮细胞中得到了表达,绿色荧光分布在细胞核和细胞质中,为进一步研究新基因的亚细胞定位和瞬时表达奠定了基础.
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| 关 键 词： | 根癌农杆菌 洋葱表皮细胞 双元表达载体 GFP基因 |
Localization Analysis of GFP in Onion Epidermal Cell via Agrobacterium tumefaciens-Mediated Transformation |
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| LIU Xiao-fei,LIANG Wei-hong.Localization Analysis of GFP in Onion Epidermal Cell via Agrobacterium tumefaciens-Mediated Transformation[J].Journal of Henan Normal University(Natural Science),2009,37(1). |
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| Authors: | LIU Xiao-fei LIANG Wei-hong |
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| Institution: | College of Life Sciences;Henan Normal University;Xingxiang 453007;China |
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| Abstract: | The plant transformation binary vector pCAMBIA1300-35S-GFP harboring a CaMV 35S promoter fused green fluorescent protein(GFP)reporter gene has been constructed,and transformed into onion epidemal cells by Agrobacterium-mediated method.Fluorescence microscope observation show that the reporter GFP gene expresses in onion epidermal cells both in cytoplasm and nucleus.The result fasciliate the further study on subcellular localization and transient expression of new genes in onion epidermal cells. |
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| Keywords: | Agrobacterium tumefaciens onion epidermal cells binary vector GFP gene |
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