A rotating bottle culture method with continuous replacement of the gas phase |
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Authors: | D. A. T. New D. L. Cockroft |
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Affiliation: | (1) Physiological Laboratory, University of Cambridge, CB2 3EG Cambridge, (England) |
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Abstract: | Summary A rotator culture method is described which provides a continuous flow of oxygenating gas to cultures in rotating bottles. The system maintains constant O2 and CO2 levels in the culture medium throughout the incubation period. It also provides a more stable pH than systems with sealed culture bottles.Acknowledgments. We would like to thank Mr B. Secker, Mr C. Hellon and Mr S. J. Ellis, of the Physiological Laboratory workshop, for constructing the rotator apparatus. We also thank the Medical Research Council, London, for financial support for the project. |
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