黄孢原毛平革菌(Phanerochaete chrysosporium)厚垣孢子降解阿特拉津的探究

阿特拉津(atrazine)是一类普遍存在于环境中且难降解的污染物.本文探究了黄孢原毛平革菌(Phanerochaete chrysosporium)厚垣孢子对阿特拉津降解的最佳条件,包括温度、摇床转速、初始培养基pH及接种量.并在大田土壤盆栽实验中,研究P.chrysosporium厚垣孢子和土壤土著微生物对土壤中阿特拉津的降解情况.结果表明:P.chrysosporium厚垣孢子可以有效去除阿特拉津,在33℃、转速为180r·min~(-1)、pH值为7.0、接种量是4g·L~(-1)时,去除效果最好,去除率达90.77%.土壤盆栽实验结果表明:施用P.chrysosporium厚垣孢子28d后,非灭菌土壤中阿特拉津去除率为97.8%,其中P.chrysosporium的降解贡献最为突出,去除能力为59.3%.而土著土壤微生物的去除率仅为20.7%,表明P.chrysosporium厚垣孢子对AT降解效果明显.

The Bio-degradation of Atrazine by Chlamydospore of Phanerochaete chrysosporium

Atrazine is one kind of refractory organics extensively existing in the environment.In the study,results showed that chlamydospore of Phanerochaete chrysosporium can significantly degrade Atrazine in both laboratory studies and field trials.The highest degradation rate reached 90.77% with the optimal conditions as following:33 ℃,180r·min~(-1),pH7.0and 4g·L~(-1) inoculum quantity into a 250mL/500 mL flask.When chlamydospore of Phanerochaete chrysosporium was applied to the soil,the degradation rate was 97.8% while control soil without chlamydospore was 20.7%.The pot cultivation experiments showed that the degradation rate reached 97.8%after Phanerochaete chrysosporiumchlamydospore was applied to sterilized soil for 28 days.This suggests that chlamydospore of Phanerochaete chrysosporium is the main factor during the whole degradation process in field conditions.The degradation rate of Phanerochaete chrysosporium was 59.3% while indigenous soil microbial was only 20.7%.In summary,chlamydospore of Phanerochaete chrysosporiumcan degrade Atrazine effectively and has a potential use in the area of environment protection.