| 激光诱导沙田柚变异的RAPD研究 |
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| 引用本文： | 钱吉,谌敦华,陈燃,李斌,郑师章,李庄,韦文楼,陈茂鑫.激光诱导沙田柚变异的RAPD研究[J].复旦学报(自然科学版),2000,39(3):327-330. |
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| 作者姓名： | 钱吉 谌敦华 陈燃 李斌 郑师章 李庄 韦文楼 陈茂鑫 |
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| 作者单位： | 1. 复旦大学生物多样性科学研究所
2. 广西大学物理系 |
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| 摘 要： | 选用经初步筛选的２０个短序列引物（１０ｎｔ）和随机抽取的２个随机长序列引物，对激光诱导产生的无核沙田柚（处理组）及未经激光处理的有核沙田柚（对照组）进行了ＲＡＰＤ（随机扩增多态性ＤＮＡ）分析。结果有６个引物扩增出了共２０条多态性片段，从而表明在沙田柚的处理组与对照组之间，存在ＤＮＡ分子水平上差异，也就是说在激光诱导沙田柚无核化的过程中，已经引起了沙田柚后代基因组的显著变异，且这种诱导所引起的性状改
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| 关 键 词： | 沙田柚 RAPD 遗传变异 激光诱导 |
| 文章编号： | 0427-7104(2000)03-0327-04 |
RAPD Study on Laser-Mutagenesis Shatian Pumelo |
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| QIAN Ji,CHEN Dun-hua,CHEN Ran,LI Bing,ZHENG Shi-zhang,LI Zhuang,WEI Wen-lon,CHEN Mao-xin.RAPD Study on Laser-Mutagenesis Shatian Pumelo[J].Journal of Fudan University(Natural Science),2000,39(3):327-330. |
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| Authors: | QIAN Ji CHEN Dun-hua CHEN Ran LI Bing ZHENG Shi-zhang LI Zhuang WEI Wen-lon CHEN Mao-xin |
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| Institution: | 1.Fudan University; 2. Guangxi University |
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| Abstract: | Twenty short arbitrary primers (10 nt) and two long ones (20 nt) were used in random amplified polymorphic DNA (RAPD) analysis for different strains of Shatian Pumelo. Among them, six primers could generate twenty polymorphic bands. With RAPD method, some genetic variations were shown between the Shatian Pumelo strains irradiated by laser and the wild strain. Further analysis of RAPD demonstrated that laser irradiation can induce the genetic variation of Shatian Pumelo. |
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| Keywords: | Shatian Pumelo RAPD genetic variation |
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