Genetic dissection of a behavioral quantitative trait locus shows that Rgs2 modulates anxiety in mice |
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Authors: | Yalcin Binnaz Willis-Owen Saffron A G Fullerton Jan Meesaq Anjela Deacon Robert M Rawlins J Nicholas P Copley Richard R Morris Andrew P Flint Jonathan Mott Richard |
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Institution: | Wellcome Trust Centre for Human Genetics, University of Oxford, Roosevelt Drive, Oxford OX3 7BN, UK. |
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Abstract: | Here we present a strategy to determine the genetic basis of variance in complex phenotypes that arise from natural, as opposed to induced, genetic variation in mice. We show that a commercially available strain of outbred mice, MF1, can be treated as an ultrafine mosaic of standard inbred strains and accordingly used to dissect a known quantitative trait locus influencing anxiety. We also show that this locus can be subdivided into three regions, one of which contains Rgs2, which encodes a regulator of G protein signaling. We then use quantitative complementation to show that Rgs2 is a quantitative trait gene. This combined genetic and functional approach should be applicable to the analysis of any quantitative trait. |
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