| 鲇和大口鲇同工酶比较研究 |
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| 引用本文： | 方平,王志坚,高峰.鲇和大口鲇同工酶比较研究[J].西南师范大学学报(自然科学版),2002,27(4):572-575. |
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| 作者姓名： | 方平 王志坚 高峰 |
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| 作者单位： | 1. 涪陵师范学院生命科学系,重庆,408003
2. 西南师范大学生命科学学院,重庆,400715 |
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| 摘 要： | 采用聚丙烯酰胺垂直平板凝胶电泳技术，对鲇和大口鲇的肌肉、心脏、肝脏、肾脏、脑、眼等6种组织器官乳酸脱氢酶（LDH）和酯酶（EST）同工酶进行了比较研究。结果表明：2种鲇的同种组织中乳酸脱氢酶（LDH）同工酶的酶谱相同，但在不同组织中表现出明显的组织特异性；而2种鲇的酯酶（EST）同工酶谱表现出明显的组织特异性和物种特异性。笔者还对2种鲇的亲缘关系进行了探讨。
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| 关 键 词： | 比较研究 大口鲇 鲇 乳酸脱氢酶 酯酶 同工酶 组织特异性 物种特异性 |
| 文章编号： | 1000-5471(2002)04-0572-04 |
| 修稿时间： | 2001年7月2日 |
Comparative Studies on LDH and EST Isozymes in Silurus meridionalis Chen and Silurus asotus L. |
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| FANG Ping\,\ WANG Zhi jian\,\ GAO\ Feng\ Dept. of Biology,Fuling Normal University,Chongqing ,China, School of Life Sciences,Southwest China Normal University,Chongqing ,China.Comparative Studies on LDH and EST Isozymes in Silurus meridionalis Chen and Silurus asotus L.[J].Journal of Southwest China Normal University(Natural Science),2002,27(4):572-575. |
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| Authors: | FANG Ping\ \ WANG Zhi jian\ \ GAO\ Feng\ Dept of Biology Fuling Normal University Chongqing China School of Life Sciences Southwest China Normal University Chongqing China |
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| Institution: | FANG Ping\+1,\ WANG Zhi jian\+2,\ GAO\ Feng\+2 1 Dept. of Biology,Fuling Normal University,Chongqing 408003,China, 2 School of Life Sciences,Southwest China Normal University,Chongqing 400715,China |
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| Abstract: | The electrophoretogram of LDH and EST isozymes in six tissues (heart,brain, eye, muscle, kidney, liver) of Slurus meridionalis Chen and Silurus asotus Linnaeus were analyzed by vertical polyacrylamide gel electrophoresis. The results showed that the electrophoretograms of LDH isozymes in the same tissues of the two species catfishes were similar, but they had different pattens in different tissues, and the electrophoretograms of EST isozymes had obvious tissues and species specificity. Additional the relationship of the two species catfishes was discussed. |
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| Keywords: | Silurus meridionalis Chen Silurus asotus Linnaeus Lactate dehydrogenase(LDH) Esterase(EST) Isozymes |
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