| 栗叶多糖的分离、纯化及抗氧化活性(英文) |
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| 引用本文： | 王新,孔玉梅,刘彬,王君.栗叶多糖的分离、纯化及抗氧化活性(英文)[J].辽宁大学学报(自然科学版),2011,38(1):54-59. |
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| 作者姓名： | 王新 孔玉梅 刘彬 王君 |
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| 作者单位： | 1. 辽宁大学,药学院,辽宁沈阳110036
2. 辽宁大学,化学院,辽宁沈阳,110036 |
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| 基金项目： | 辽宁省教育厅科学研究计划资助 |
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| 摘 要： | 从栗叶中分离纯化得到多糖并研究其体外抗氧化活性。从栗叶中提取得到栗叶粗多糖CLP。CLP经DE-52纤维素柱分离纯化后,于蒸馏水洗脱液中得到一个纯化多糖组分CLP1。采用体外试验研究了CLP与CLP1对羟自由基、超氧阴离子自由基、过氧化氢的清除能力及还原能力。结果表明,CLP与CLP1均具有一定的清除羟自由基、超氧阴离子自由基、过氧化氢的能力及还原能力,而且与多糖的浓度成正相关性。其中,CLP对过氧化氢的清除作用效果与VC相当。说明CLP与CLP1可以开发用作天然抗氧化剂。
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| 关 键 词： | 栗叶 多糖 纯化 抗氧化活性 |
Isolation,Purification and antioxidant activities of polysaccharides from the leaves of Castanea mollissima Bl. |
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| WANG Xin,KONG Yu-mei,LIU Bin,WANG Jun.Isolation,Purification and antioxidant activities of polysaccharides from the leaves of Castanea mollissima Bl.[J].Journal of Liaoning University(Natural Sciences Edition),2011,38(1):54-59. |
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| Authors: | WANG Xin KONG Yu-mei LIU Bin WANG Jun |
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| Institution: | WANG Xin1,KONG Yu-mei1,LIU Bin1,WANG Jun2(1.School of Pharmaceutical Sciences,Liaoning University,Shenyang 110036,China,2.College of Chemistry,China) |
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| Abstract: | The polysaccharides were isolated and purified from the leaves of Castanea mollissima Bl.and their antioxidant activities were studied in vitro.Crude polysaccharides(CLP) was isolated from the leaves of Castanea mollissima Bl.CLP was purified by applying a cellulose DE-52 column,and the polysaccharides obtained from the eluent with water were termed as CLP1.The antioxidant activities of CLP and CLP1,such as scavenging ability to hydroxyl radicals,superoxide radicals and hydrogen peroxide,and reducing power ... |
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| Keywords: | the leaves of Castanea mollissima Bl polysaccharide purification antioxidant activities |
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