| 酿酒酵母与雨生红球藻的细胞融合子的RAPD鉴定 |
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| 引用本文： | 江胜滔,施巧琴,黄建忠,王明兹,施碧红,林跃鑫.酿酒酵母与雨生红球藻的细胞融合子的RAPD鉴定[J].福建师范大学学报(自然科学版),2004,20(4):76-79. |
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| 作者姓名： | 江胜滔 施巧琴 黄建忠 王明兹 施碧红 林跃鑫 |
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| 作者单位： | 福建师范大学生物工程学院,福建,福州,350007 |
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| 基金项目： | 福建省计划委员会基金重点资助项目(IC0001) |
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| 摘 要： | 用7条随机引物对雨生红球藻(Haemotococcumpluvies)、酿酒酵母(Saccaromycescerevisiae)及其融合酵母变株进行RAPD分析.在融合子的54条谱带中,有7条为双亲共有,占12.96%,与酿酒酵母相同的18条(33%),与雨生红球藻相同的17条(30.9%),其余12条(23.54%)为融合子的新谱带,结果表明该融合子很可能是雨生红球藻、酿酒酵母细胞杂种.
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| 关 键 词： | 雨生红球藻 酿酒酵母 融合酵母变株 RAPD |
| 文章编号： | 1000-5277(2004)04-0076-04 |
| 修稿时间： | 2004年5月16日 |
RAPD Identification of the Protoplast Fusant of Haemotococcum pluvies and Saccaromyces cerevisiae |
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| JIANG Sheng-tao,SHI Qiao-qin,HUANG Jian-zhong,WANG Ming-zi,SHI Bi-hong,LIN Yue-xin.RAPD Identification of the Protoplast Fusant of Haemotococcum pluvies and Saccaromyces cerevisiae[J].Journal of Fujian Teachers University(Natural Science),2004,20(4):76-79. |
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| Authors: | JIANG Sheng-tao SHI Qiao-qin HUANG Jian-zhong WANG Ming-zi SHI Bi-hong LIN Yue-xin |
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| Abstract: | Haemotococcum pluvies, Saccaromyces cerevisiae and their protoplast fusant were tested by RAPD method with 7 randomly selected primers. Among 54 electrophoresis bands of the fusant, 7 bands can be found in both parents (12.96%), 18 bands can be found in one of the parent Saccaromyces cerevisiae (33.0%), 17 bands can be found in another parent Haemotococcum pluvies (30.9%). 12 bands are brand-new ones which can not be found in both parents. The results indicated that the fusant are possibly the result of protoplast fusing hybrid of Haemotococcum pluvies and Saccaromyces cerevisiae. |
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| Keywords: | Haemotococcum pluvies Saccaromyces cerevisiae protoplast fusant RAPD |
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