| 结合叶绿体基因matK/trnK序列和生境分析不同生态型芦苇的系统发育 |
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| 引用本文： | 龚晓洁,闫广为,浦铜良.结合叶绿体基因matK/trnK序列和生境分析不同生态型芦苇的系统发育[J].兰州大学学报(自然科学版),2009,45(3). |
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| 作者姓名： | 龚晓洁 闫广为 浦铜良 |
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| 作者单位： | 兰州大学生命科学学院植物学及植物生理研究所,兰州,730000 |
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| 摘 要： | 以甘肃省临泽县境内的4种生态型芦苇(Phragmites communis Trin.)的18个样品为材料,采用PCR扩增技术,以通用引物"matK-FF74"和"matK-trnK-2R"扩增matK/trnK序列,对纯化后的产物进行序列测定和分析.序列比对软件为Chistal W,系统发育软件分析为PAUP4b10并用以构建MP和ML发育树和计算遗传距离,外群为芦竹(Arundo donax).结果表明:matK/trnK序列长为1745～1753 bp,含有91个简约信息位点和120个可变位点,获得3个严格一致树.MP树和ML树共同说明水生芦苇是最古老的类群,它与沙丘芦苇之间的遗传距离最远,重度盐渍过渡型芦苇和轻度盐渍过渡型芦苇属于中间过渡类群.
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| 关 键 词： | 生态型芦苇 系统发育 遗传距离 最大简约法 最大似然法 甘肃省临泽县 |
Analysis of phylogeny of different ecotypes of reed(Phragmites communis Trin.) based on chloroplast DNA matK/trnK sequence and ecological environment |
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| GONG Xiao-jie,YAN Guang-wei,PU Tong-liang.Analysis of phylogeny of different ecotypes of reed(Phragmites communis Trin.) based on chloroplast DNA matK/trnK sequence and ecological environment[J].Journal of Lanzhou University(Natural Science),2009,45(3). |
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| Authors: | GONG Xiao-jie YAN Guang-wei PU Tong-liang |
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| Institution: | Institute of Botany and Plant Physiology;School of Life Sciences;Lanzhou University;Lanzhou 730000;China |
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| Abstract: | 18 reed samples of four ecotypes(Phragmites communis Trin.) from Linze County,Gansu Province were used as materials and their DNA were isolated with CTAB methods.matK/trnK sequences of chloroplast genome were amplified with primer pairs named matK-FF74 and matK-trnK-2R,then PCR products were sequenced and analysed by Clustal W software and PAUP4b10 software.Analysis methods were maximum parsimony method and maximum likelihood method.The data were used for reconstructing the phylogenetic trees and calculatin... |
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