甘油-3-磷酸对甲肝疫苗诱导的体液免疫影响*

目的 研究甘油-3-磷酸对甲肝疫苗诱导的小鼠体液免疫应答的影响。方法 选取49只雌性ICR小鼠，分7组，每组7只，这7组小鼠分别为阴性对照、单纯抗原组、铝佐剂组和4组甘油-3-磷酸组，分别在免疫之后的第4、8、12、16、20周用酶联免疫法(ELISA)测定小鼠血清中的特异性的anti-HAV IgG水平。结果 在小鼠免疫之后的第4、8、12、16、20周单纯抗原组、铝佐剂组和4组不同剂量甘油-3-磷酸组均能检测到特异性抗HAV抗体，并且趋势为先升高后降低，且在免疫后的第8周最高；第4周和第8周的甘油-3-磷酸实验组的结果均高于单纯抗原组，且差异均具有统计学意义(P<0.05)，并且第4周甘油-3-磷酸组抗体水平均超过铝佐剂组，并具有统计学意义(P<0.05)，其中甘油-3-磷酸2 mg为最佳剂量组，其抗体水平在免疫后的第4、8、12、16、20周均高于单纯抗原组，且在第8周抗体水平达到峰值，抗HAV IgG水平为lg(3.064±0.07851),铝佐剂的也是在第8周抗体水平达到峰值，抗HAV IgG水平为lg(3.150 ±0.1382)。结论 甘油-3-磷酸有免疫佐剂的作用，能够有效、迅速地增强HAV诱导的体液免疫应答，是一种潜在、安全有效的新型疫苗佐剂。

Effects of Glycerol-3-phosphate on Humoral Immune Response Induced by Hepatitis a Virus Vaccine in Mice

Objective To investigate the effect of G3P(Glycerol-3-phosphate) on humoral immune response induced by hepatitis a virus vaccine in mice. Method Forty nine ICR female mice were divided randomly into 7 groups (7 mice in each group): negative control group, antigen group, aluminum adjuvant group and 4 groups of G3P. The specific anti-HAV IgG levels in the serum were detected by enzyme-linked immunosorbent assay (ELISA) at the 4th, 8th, 12th, 16th, and 20th week after immunization. Result The serum anti-HAV IgG levels were detected in antigen group, aluminum adjuvant group and 4 groups of G3P at the 4th, 8th, 12th, 16th, and 20th week after immunization, and the serum IgG level of the mice increased first and then decreased, and the level reached the highest at 8th week. The serum IgG level of G3P group was higher than the antigen group and the difference was statistically significant (P<0.05)at 4th week and 8th week, besides, the serum IgG level of G3P group was higher than aluminum adjuvant group at 4th week and the difference was statistically significant(P<0.05).The serum IgG level of G3P 2 mg was the highest in all of G3P,and the level was higher than the antigen group at 4th, 8th, 12th, 16th, and 20th week after immunization and reached the peak at the 8th week, with the value of lg(3.064 ± 0.07851), aluminum adjuvant also reached the peak at 8th weeks, with the value of lg (3.150 ± 0.1382). Conclusion G3P could significantly and rapidly enhance humoral immune response induced by HAV vaccine in mice; the result suggest it may be used to develop a new type of human vaccine adjuvant．