冷藏雄鼠尸体应用于小鼠运输及意外死亡后品系恢复的可行性

目的 基于小鼠尸体4 ℃冷藏不同时长后精子的体外受精(IVF)效率，探讨低温冷藏小鼠尸体应用于小鼠运输及意外死亡后品系恢复的可行性。方法 实验组为5月龄C57BL/6 J雄鼠，脱颈处死后4 ℃冷藏24、48、72 h和96 h，取附睾尾精子IVF，对照组为正常5月龄C57BL/6 J雄鼠IVF，统计各组IVF受精率。结果 冷藏24 h后附睾尾精子具备正常受精能力，受精率为73.0%、60.0%和77.0%；48 h 组内差异大，受精率为0%、55.4%和3.2%；72 h受精率极低，受精率为3.2%、0%和1.7%；96 h 精子有活力，但无受精能力。所有受精卵均可发育至2细胞，冷藏24 h组和对照组分别移植受体妊娠率分别为60%和66%，证明4 ℃冷藏对胚胎发育无影响。结论 C57BL/6J鼠经4 ℃冷藏的附睾尾，在24 h内具备稳定的受精率，可用于小鼠运输及特殊情况品系恢复。

he Feasibility of Recovering Strains Used for Transportation and Accidental Death of the Mice by Refrigerating Male Mouse Body

To investigate the feasibility of recovering strains used in the transportation and accidental death of the mice by refrigerating male mouse body at low temperature, based on the efficiency of in vitro fertilization (IVF) at different time length of mice were refrigerated at 4 ℃. All experimental C57BL/6 J male mice were 5 months old. Taking the mice cauda epididymis sperm fertilization in vitro after executed and refrigerated at 4 ℃ in 24 h, 48 h, 72 h and 96 h. The experiment of C57BL/6 J male mice executed and fertilization in vitro as the Control group. Statistical groups of the fertilization rate were calculated by groups. After 24 hours of refrigerated, the spermatozoa of the cauda epididymides had the capacity of normal fertilization, and the fertilization rates were 73.0%, 60.0% and 77.0%. There was the obvious difference within the group after 48 hours of refrigerated. The fertilization rates were 0%, 55.4% and 3.2%. After 72 hours of refrigerated, the fertilization rate was 3.2%, 0%, and 1.7%. After 96 hours of refrigerated, The sperm vitality, but no fertilization ability. All fertilized eggs can develop to 2-cell embryos. The pregnancy rates were 60% and 66% respectively by transplanting the 2-cell embryos of 24 h group and control group. It was proved that there was no effect on the early development of fertilized eggs at 4 ℃.C57BL/6 J mice had the stability of the fertilization rate in 24 h at 4 ℃, can be used for transporation and strain recovery after accidental death.