四氯化碳诱导近交系C57BL/6小鼠建立肝纤维化模型

目的 比较不同剂量四氯化碳诱导近交系C57BL/6小鼠肝纤维化模型的效果，以建立稳定的肝纤维化模型。方法 选择5周龄的C57BL/6近交系小鼠，分别腹腔注射给予高、中、低剂量的四氯化碳诱导肝纤维化，实验结束后取血及肝脏组织，检测血浆丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)的水平及肝脏病理变化。结果 近交系C57BL/6小鼠药物诱导后，各剂量组动物均能存活到8周，高剂量组存活率仅为20%远低于中、低组；血清转氨酶含量升高程度、肝组织病理改变存在剂量时间效应关系。病理分析表明，各剂量组均可见肝细胞变性/坏死、中央静脉周围/汇管区混合细胞浸润及肝脏纤维化。结论 10%四氯化碳给药8周后能诱导近交系C57BL/6小鼠形成较稳定的肝纤维小鼠模型，为后续肝纤维化机制研究及药物筛选工作奠定了基础。

Establishment of Hepatic Fibrosis Model Induced by CCl4 in Inbred C57BL/6 Mice

Objective Compared the effects of different doses of carbon tetrachloride induced liver fibrosis in inbred C57BL/6 mice, in order to established a stable liver fibrosis model for the study of liver diseases and related drug mechanisms. Method The 5-week-old C57BL/6 mice were subjected to high, medium and low dose by CCl4, after the experiment collected blood and liver tissue to detect the levels of plasma alanine aminotransferase (ALT), aspartate aminotransferase (AST) and liver pathology. Result After the induction of inbred C57BL/6 mouse drug, the medium and low dose group could survive for 8 weeks, the increase of serum transaminase content and the pathological changes of liver tissues had a dose-time effect, and the mice with severe pathological changes had a large area of liver tissue necrosis and were wrapped by fibrous tissue. Conclusion Eight weeks after administration of 10% carbon tetrachloride, inbred C57BL/6 mice were induced to form a stable mouse model of liver fiber.