STR扫描技术对融水小型猪群体遗传结构的分析*

目的 应用STR扫描技术分析融水小型猪群体的遗传结构。方法 选用北京市地方标准封闭群实验用小型猪的遗传质量监测提供的25个微卫星位点对广东省医学实验动物中心小型猪研究基地F1代30头融水小型猪基因组DNA进行PCR扩增，对扩增产物进行STR扫描，运用popgene32软件对该融水小型猪进行群体遗传结构分析。结果 融水小型猪F1群体平均观测等位基因数为9.4400，平均有效等位基因数为5.2966，平均香隆指数为1.8085，平均有效杂合度为0.7737。结论 STR扫描技术适用于融水小型猪群体遗传结构分析；初步掌握了融水小型猪遗传结构。

Analyzsis of Population Genetic Structure of Rongshui Miniature Pig by STR Scan Technology

To evaluate the genetic structure of Rongshui miniature pig by STR scan technology. Twenty five microsatellite sites selected Beijing local standards were applied to amplify the genomic DNA from 30 Rongshui miniature pig for F1 generation in the miniature pig base of Guangdong medical laboratory animal center by PCR, the PCR product were analyze by STR scanning, software popgene 32 was used to analyze the genetic structure. For the Rongshui miniature pig population for F1 generation, the average observed number of alleles is 9.4400, the average effective number of alleles is 5.2966, the average shannon index is 1.8085, and the average effective heterozygosity is 0.7737. The STR scan technology can be application in genetic structure analysis of miniature pig population, and we initially grasp the genetic structure of Rongshui miniature pig