IVC笼盒原位实施小鼠二氧化碳安乐死方法的建立

目的 建立一种在原饲养IVC笼盒中实施小鼠二氧化碳安乐死的方法。方法 将4～5周龄BALB/c小鼠、4～5周龄C57BL/6小鼠和2周龄C57BL/6小鼠各10只，按照5只/笼的标准饲养。以28%容器体积/min气体置换率，将二氧化碳通入小鼠饲养笼实施安乐死。结果 4～5周龄BALB/c小鼠和C57BL/6小鼠均在2～3 min之间失去意识，5～6 min之间死亡，与美国国立卫生研究院指南中要求的一致。2周龄C57BL/6小鼠死亡需要的时间长于4～5周龄C57BL/6小鼠，并有显著性差异。结论 成功建立了小鼠在原饲养IVC笼盒中实施二氧化碳安乐死的方法。该方法有效降低了小鼠应激反应，提高了动物福利；且有利于操作人员身心健康。

Establishment of a Carbon Dioxide Euthanasia Method Based on Mice Individually Ventilated Cage

Objective To establish a carbon dioxide euthanasia method based on mice individually ventilated cage. Method Four to five weeks old BALB/c mice, C57BL/6 mice and 2 weeks old C57BL / 6 mice were respectively killed in their individually ventilated cages with carbon dioxide (the fill rate was 28% chamber volume per minute). Result Four-five weeks old BALB/c mice and C57BL/6 mice lost consciousness whithin 2-3 min and died whithin 5-6 min, which meets the requirement of NIH guidelines. Two weeks old C57BL/6 mice took 6-8 minutes before death, which has significant difference comparing with 4-5 weeks old C57BL/6 mice. Conclusion successfully established a carbon dioxide euthanasia method based on mice individually ventilated cage. This method effectively reduces the stress reaction in mice, and contributes to physical and mental health of operators.