慢性尿路感染大鼠模型的动态变化研究

目的 建立慢性尿路感染大鼠模型并研究相关指标的动态变化。方法 雌性SD大鼠经膀胱处注入大肠杆菌液，以诱导慢性尿路感染大鼠模型，并灌胃盐酸左氧氟沙星治疗。第0、7、14、21、28天动态检测动物体温、尿细菌数、血常规及BUN、Cr值的变化。第31天进行肾脏及膀胱感染菌计数及组织病理学检测。结果 模型对照组在第7、14天体温显著升高(P<0.05)；第7～28天尿细菌数、第30天肾脏及膀胱感染菌数明显增多(P<0.05)；第30天膀胱及肾脏病理检查可见炎性细胞浸润及黏膜下层血管扩张充血。盐酸左氧氟沙星组尿细菌数、肾脏及膀胱染菌数明显下降(P<0.05)，膀胱及肾脏炎症明显减轻。结论 此法适用于建立慢性尿路感染大鼠模型及进行药效学观察。

Animal Experiment Research on the Dynamic Change of Chronic Urinary Tract Infection Disease

In order to establish the animal model of chronic urinary tract infection(CUTI)and study the dynamic changes of the relevant indicators, the CUTI model was induced by injecting Escherichia coli into the bladder of the female rat, and treated with levofloxacin hydrochloride at the same time. Indicators such as body temperature, number of urine bacteria, blood routine and BUN, Cr value were detected on day 0, 7, 14, 21 and 28. On day 31, number of bacterium in kidney and bladder was counted and histopathological examination was performed. Under the study, we discovered that on day 7 and 14, the body temperature elevated significantly(P<0.05)and the number of bacterium in urine, kidney and bladder increased obviously(P<0.05)in the model group rather than in the sham-operated group. Inflammation of bladder and kidney could be observed either. However, administrated with levofloxacin hydrochloride, the number of bacterium was significantly decreased no matter in urine or bladder or kidney. What’s more, inflammation of bladder and kidney were attenuated. In a word, the method carried out in this study is suitable for building CUTI animal model and pharmacodynamics observation.