一株分离自艾丁湖土壤的嗜盐古菌新种的研究

为了研究分析新疆盐湖嗜盐古菌资源，从艾丁湖中分离筛选出一批嗜盐古菌，对其进行了形态特征，生理生化特性研究和分子鉴定。发现其中1株菌（D -20）的生理生化特性和产淀粉酶特性比较突出，经不同温度，不同pH，不同温度下的研究以及16S rDNA 序列的系统发育学研究表明：该菌株为革兰氏染色阴性菌，最适生长温度为37℃，最适生长pH8.0，最适盐浓度15％，能产过氧化氢酶、尿酶，淀粉酶，硝酸盐还原为阳性，不产 H2S ，对青霉素，氯四环素，红霉素，新霉素具有抗性，能利用赖氨酸，蛋氨酸，精氨酸，以果糖，蔗糖和核糖作为碳源。经分子鉴定该菌是 Natrinema属的新成员，定名为 Natrinema aidingensis D -20，Gene Bank登录号为 AY298734。菌株保藏号为12．657。

The Study of Self and Soilhalophilic Archaea Species Separation Plant

In order to analysis of halophilic archaea from Xinjiang Saline Lake resources research ,se-lected a group of halophilic archaea isolated from Aydingkol lake ,its morphological characteristics ,physi-ological and biochemical characteristics and molecular identification .Found that 1 strains of bacteria （D -20） on physiological and biochemical characteristics and amylase producing conspicuous characteristics , with different temperature ,different pH ,studies show that growth under different temperature and 16S rDNA sequence of the system ：the strain is gram negative bacteria ,optimum growth temperature was 37℃ ,the optimum growth pH 8 ,the most salt concentration 15% ,can produce catalase ,urease ,amylase , nitrate reduction is positive ,do not produce H2S ,penicillin ,tetracycline ,erythromycin ,neomycin resist-ant ,can make use of lysine ,methionine ,arginine ,fructose ,sucrose and ribose as carbon source .The strain D - 20 is a novel member of the Natrinema aidingensis of Bank , Gene accession number is AY298734 .