| 以提高乙肝病毒表面抗原表达量和免疫原性为目的的番茄果实特异表达载体的构建 |
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| 引用本文： | 祁瑜,贺竹梅,郑诺燕,徐茵.以提高乙肝病毒表面抗原表达量和免疫原性为目的的番茄果实特异表达载体的构建[J].中山大学学报(自然科学版),2005,44(6):75-78. |
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| 作者姓名： | 祁瑜 贺竹梅 郑诺燕 徐茵 |
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| 作者单位： | 中山大学生命科学学院,广东,广州,510275 |
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| 摘 要： | 为了提高乙肝病毒表面抗原基因在番茄果实中的表达量和免疫原性,采用克隆的E8番茄果实特异表达启动子和乙肝病毒表面抗原M蛋白基因构建了植物表达载体pBIES2, 及只含有乙肝病毒表面抗原S蛋白的表达载体pBIES,并将它们分别导入根癌农杆菌LBA4404中.最后对该载体的优越性进行了讨论.
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| 关 键 词： | E8启动子 乙肝病毒表面抗原 植物表达载体 |
| 文章编号： | 0529-6579(2005)06-0075-04 |
| 收稿时间： | 04 13 2005 12:00AM |
| 修稿时间： | 2005年4月13日 |
Construction of Two Plant Expression Vectors for Enhancing Expression and Immunogenicity of HBsAg in Tomato |
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| QI Yu,HE Zhu-mei,ZHENG Nuo-yan,XU Yin.Construction of Two Plant Expression Vectors for Enhancing Expression and Immunogenicity of HBsAg in Tomato[J].Acta Scientiarum Naturalium Universitatis Sunyatseni,2005,44(6):75-78. |
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| Authors: | QI Yu HE Zhu-mei ZHENG Nuo-yan XU Yin |
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| Institution: | School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China |
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| Abstract: | Expression amount and immunogenicity of plant-produced vaccine are two main problems in the development of plant-based vaccine. For the purpose of enhancing expression in tomato fruits and immunogenicity of HBsAg, we constructed plant expression vector pBIES2, which contains HBsAg M-protein gene and under the control of E8 tomato fruit-specific promoter. Another expression vector pBIES, containing HBsAg S-protein gene and under the control of E8 promoter, was constructed as well. Both the vectors were introduced into A. tumefaciens LBA4404. A brief discussion for the advantages of pBIES2 was processed at the end of the article. |
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| Keywords: | E8 promoter HBsAg plant expression vector |
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