| 逆转录病毒载体设计对ANTI—RAS核酶基因转移效率的影响 |
| |
| 引用本文： | 赵立青,张丽.逆转录病毒载体设计对ANTI—RAS核酶基因转移效率的影响[J].南开大学学报,1997,30(4):8-13. |
| |
| 作者姓名： | 赵立青 张丽 |
| |
| 作者单位： | 南开大学分子生物学研究所!天津，300071 |
| |
| 基金项目： | 天津市21世纪-青年科学基金 |
| |
| 摘 要： | 将ａｎｔｉ－ｒａｓ核酶基因Ｒ８克隆于不同的逆转录病毒载体，并导入逆转录病毒包装细胞质ＰＡ３１７，得到具一次感染性的缺失性病毒，通过测定病毒滴度选择Ｒ８基因的高效重组转录病毒载体。
|
| 关 键 词： | 逆转录病毒载体 核酶 基因转移 载体设计 |
THE EFFECT OF DESIGN OF RETROVIRUL VECTORS ON THE TRANSFER EFFICENCY OF ANTI-RAS RIBOZYME GENE |
| |
| Zhao Liqing, Zhang Li, Chen Yawen, Shi Guoli,You Huanyu, Chen Defeng.THE EFFECT OF DESIGN OF RETROVIRUL VECTORS ON THE TRANSFER EFFICENCY OF ANTI-RAS RIBOZYME GENE[J].Acta Scientiarum Naturalium University Nankaiensis,1997,30(4):8-13. |
| |
| Authors: | Zhao Liqing Zhang Li Chen Yawen Shi Guoli You Huanyu Chen Defeng |
| |
| Abstract: | A series of recombiant retroviral vectors which contain riobzyme R8 gene were constructed, then transfected packing cell lines PA317 to get retrovial vector producer cell clones. The most effcient vector was got by testing the titer of the recombinat virus. |
| |
| Keywords: | retroviral vector ribosome gene transfer titer |
| 本文献已被 CNKI 维普 等数据库收录！ |
