| KK-42对日本沼虾蜕皮激素及其受体表达的影响 |
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| 引用本文： | 刘方,陈香丽,郭爱莲,宁黔冀.KK-42对日本沼虾蜕皮激素及其受体表达的影响[J].河南师范大学学报(自然科学版),2013,41(4):124-127. |
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| 作者姓名： | 刘方 陈香丽 郭爱莲 宁黔冀 |
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| 作者单位： | 河南师范大学生命科学学院,河南新乡,453007 |
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| 基金项目： | 河南省高校科技创新人才支持计划 |
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| 摘 要： | 用1.95×10-4 mol.L-1的KK-42处理日本沼虾1min,不同时刻抽取血淋巴、解剖肝胰腺.HPLC测定血淋巴中20E滴度,RT-PCR测定EcR和RXR mRNA水平.结果:KK-42处理后3h,6h,20E滴度分别比对照组增加了121.71%(P<0.05)、268.96%(P<0.01);EcR mRNA水平在处理后6h,12h,分别比对照组提高了233.56%(P<0.01)、110.73%(P<0.05);RXR mRNA水平在处理后3h、12h,分别比对照组提高了195.53%(P<0.01)、80.83%(P<0.05).表明KK-42可显著提高血淋巴中20E的滴度,并诱导肝胰腺中EcR和RXR的基因转录.
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| 关 键 词： | KK-42 日本沼虾 20E EcR RXR |
Effect of KK-42 on Ecdysone and Ecdysteroid Receptor mRNA in Macrobrachium Nipponense |
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| LIU Fang
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CHEN Xiangli
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GUO Ailian
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NING Qianji.Effect of KK-42 on Ecdysone and Ecdysteroid Receptor mRNA in Macrobrachium Nipponense[J].Journal of Henan Normal University(Natural Science),2013,41(4):124-127. |
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| Authors: | LIU Fang CHEN Xiangli GUO Ailian NING Qianji |
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| Institution: | (College of Life Sciences,Henan Normal University,Xinxiang 453007,China) |
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| Abstract: | The spawns were soaked for 1min in KK-42solution at a concentration of 1.95×10-4 mol.L-1,and then hemolymph was extracted,and hepatopancreas were removed.Hemolymph 20Etiter was determined by HPLC.EcR and RXR mRNA level were assayed by RT-PCR.The result showed that the titers of hemolymph 20Esignificantly increased after KK-42treatment compared with the corresponding controls at 3h(P<0.05) and 6h(P <0.01);the hepatopancreas EcR mRNA level significantly increased compared with the corresponding controls at 6h(P<0.01) and 12h(P<0.05) after KK-42treatment;the hepatopancreas RXR mRNA level significantly increased compared with the corresponding controls at 3h(P<0.01) and 12h(P<0.05) after KK-42treatment.It indicates that KK-42treatment enhanced ecdysone titer,and up-regulatecl the expressions of EcR and RXR. |
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| Keywords: | KK-42 Macrobrachium nipponense 20 E EcR RXR |
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