CD20-related signaling pathway is differently activated in normal and dystrophic circulating CD133+ stem cells |
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Authors: | D Parolini M Meregalli M Belicchi P Razini R Lopa B Del Carlo A Farini S Maciotta N Bresolin L Porretti M Pellegrino Y Torrente |
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Institution: | 1. Stem Cell Laboratory, Department of Neurological Sciences, Fondazione IRCCS Ospedale Maggiore Policlinico, Centro Dino Ferrari, University of Milan, via F. Sforza 35, 20122, Milan, Italy 3. Centro Interdipartimentale di Citometria, Department of Regenerative Medicine, Fondazione IRCCS Ospedale Maggiore Policlinico, via F. Sforza 35, 20122, Milan, Italy 4. Department of Human Physiology, University of Pisa, via S. Zeno 31, 56127, Pisa, Italy 2. UNISTEM, Centro Interdipartimentale di Ricerca sulle Cellule Staminali, University of Milan, via Balzaretti 9, 20133, Milan, Italy
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Abstract: | Among the heterogeneous population of circulating hematopoietic and endothelial progenitors, we identified a subpopulation
of CD133+ cells displaying myogenic properties. Unexpectedly, we observed the expression of the B-cell marker CD20 in blood-derived
CD133+ stem cells. The CD20 antigen plays a role in the modulation of intracellular calcium homeostasis through signaling pathways
activation. Several observations suggest that an increase in intracellular calcium concentration (Ca2+]i) could be involved in the etiology of the Duchenne muscular dystrophy (DMD). Here, we show that a CD20-related signaling
pathway able to induce an increase in Ca2+]i is differently activated after brain derived neurotrophic factor (BDNF) stimulation of normal and dystrophic blood-derived
CD133+ stem cells, supporting the assumption of a “CD20-related calcium impairment-affecting dystrophic cells. Presented findings
represent the starting point toward the expansion of knowledge on pathways involved in the pathology of DMD and in the behavior
of dystrophic blood-derived CD133+ stem cells.
Received 15 October 2008; received after revision 27 November 2008; accepted 05 December 2008 |
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Keywords: | " target="_blank"> Circulating stem cells CD133 CD20 Ca2+ Duchenne muscular dystrophy (DMD) brain derived neurotrophic factor (BDNF) |
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