外来入侵种空心莲子草的RAPD遗传多样性分析

基于随机扩增多态DNA(RAPD)方法,对8个居群空心莲子草Agasicles hygrophila的遗传多样性及分化程度进行分析。10条随机引物扩增出99个可分析位点,多态位点百分比为36．3％。POPOGENE分析发现,空心莲子草居群平均水平的多态位点百分比为26．2％,Nei’s基因多样度为0．0906,Shannon信息指数为0．1609,具有较高的遗传多样性;居群间遗传分化比例为30．88％,居群内遗传分化占69．12％;居群间基因流为1．1189。结合居群遗传多样性及UPGMA聚类分析表明,镇江及南京地区是空心莲子草遗传变异程度最高的中心区,应该作为外来种空心莲子草防治及根除的重点地带,防治的最有效措施是加强对其无性繁殖体临近传播、蔓延和扩散途径的阻断。

Genetic Diversity of the Invasive Plant Agasicles hygrophila Based on Random Amplified Polymorphic DNA

Genetic diversity was examined in eight populations of the invasive plant Agasicles hygrophila in Jiangsu province using random amplified polymorphic DNA(RAPD) markers.A total of 99 discernible loci was obtained for all populations using 10 primers,36.3% of which were polymorphic (P_(PL)=36.3%).As analyzed by POPOGENE,average percentage of polymorphic loc(P_(PL)=26.2%),Nei's genetic diversity(H_e=0.090 6) and Shannon's information index(H=0.160 9) indicated that A.hygrophila had a relatively high level of genetic diversity.The proportion of the genetic differentiation among the A.hygrophila populations(G_(st)) was 30.88% and the gene flow was 1.118 9.The combined information from the analyses of genetic diversity and UPGMA indicated that genetic diversity of populations in Zhenjiang and Nanjing was the highest.As the genetic structure of A.hygrophila population was related with locality,in Nanjing,a center of the transportation,we should control spreading,migration and diffusion of the asexually propagates of A.hygrophila especially.