| Expression of soluble human tumor necrosis factor receptor Ⅰ in Aspergillus niger |
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| 摘 要： | The cDNA of soluble human tumor necrosis factor receptorⅠ(sTNFRI) was inserted into fusion-protein expression plasmid pIGF of A. niger to construct fusion expression vector pHBC containing a KEX2 like protein processing site designed on the fusion position. Extracellular protease-deficient strain of A. niger 3.795-1-23 was transformed with pHBC. Positive clone was estimated by Southern hybridization. SDS-PAGE for protein produced by recombinant strain showed the distinctive expression band. Western blotting indicated that the secreted protein had immunoactivity of sTNFRI.
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| 收稿时间： | 11 December 2000 |
Expression of soluble human tumor necrosis factor receptor I inAspergillus niger |
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| Authors: | Min Li Bin Hong Yuan Li |
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| Institution: | (1) Institute of Medicinal Sciences & Peking Union Medical College, 100050 Beijing, China |
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| Abstract: | The cDNA of soluble human tumor necrosis factor receptor I (sTNFRI) was inserted into fusion-protein expression plasmid pIGF
of A. niger to construct fusion expression vector pHBC containing a KEX2 like protein processing site designed on the fusion
position. Extracellular protease-deficient strain ofA. niger 3.795-1-23 was transformed with pHBC. Positive clone was estimated by Southern hybridization. SDS-PAGE for protein produced
by recombinant strain showed the distinctive expression band. Western blotting indicated that the secreted protein had immunoactivity
of sTNFRI. |
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| Keywords: | sTNFRI Aspergillus niger KEX2 secretory expression fusion protein |
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