| 澳洲鳗、日本鳗和欧洲鳗的RAPD初步比较分析 |
| |
| 引用本文： | 胡庚东,尤洋,陈家长.澳洲鳗、日本鳗和欧洲鳗的RAPD初步比较分析[J].浙江海洋学院学报(自然科学版),2006,25(3):254-257. |
| |
| 作者姓名： | 胡庚东 尤洋 陈家长 |
| |
| 作者单位： | 中国水产科学研究院淡水渔业研究中心,江苏,无锡,214081 |
| |
| 摘 要： | 运用RAPD技术,以日本鳗Anguilla japonicus、欧洲鳗Anguilla anguilla和澳洲鳗Anguilla australia尾鳍DNA作为模板,在20种随机引物中筛选出4种随机引物进行扩增反应,得到这3种鳗鱼DNA的RAPD扩增片段,通过比较这3种鳗鱼的扩增条带的特异性、种间相似性指数(日本鳗和欧洲鳗之间的相似指数为0.556 5,日本鳗和澳洲鳗、欧洲鳗和澳洲鳗的相似指数分别为0.510 2和0.5278)和遗传距离,探讨这3种鳗鱼的遗传差异,为鳗鱼的种质鉴定奠定基础。
|
| 关 键 词： | RAPD 鳗鱼 随机引物 相似性指数 |
| 文章编号： | 1008-830X（2006）03-0254-04 |
| 收稿时间： | 2006-03-07 |
| 修稿时间： | 2006年3月7日 |
Preliminary Analysis of Genetic Variations of Three Populations in Eel by RAPD |
| |
| HU Geng-dong,YOU Yang,CHEN Jia-zhang.Preliminary Analysis of Genetic Variations of Three Populations in Eel by RAPD[J].Journal of Zhejiang Ocean University(Natural Science Edition),2006,25(3):254-257. |
| |
| Authors: | HU Geng-dong YOU Yang CHEN Jia-zhang |
| |
| Institution: | Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences,Wuxi 214081,China |
| |
| Abstract: | RAPD(random amplified polymorphic DNA) was applied to detect the genetic variation of three populations in Eel-Anguilla japonicus,Anguilla anguilla and Anguilla australia collected from Guangdong province,farmed in the ponds,about 10g.Twenty 10bp random primers were used for DNA amplification and 5 samples of each stock were analyzed.Totally 43 markers were detected in a range of 200-5 000 bp.Based on the RAPD data,the genetic similarities of them were 0.556 5 between Anguilla japonicus and Anguilla anguilla,0.510 2 between Anguilla japonicus and Anguilla australia and 0.527 8 between Anguilla anguilla and Anguilla australia.The genetic distances of them were 0.443 5 between Anguilla japonicus and Anguilla anguilla,0.4898 between Anguilla japonicus and Anguilla australia and 0.472 2 between Anguilla anguilla and Anguilla australia.They were all different. |
| |
| Keywords: | RAPD Eel random primer similarity index |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
