斜带石斑鱼转化生长因子β1（TGF β1）基因的克隆及表达分析

  转化生长因子β1（TGF β1）是一种高度保守的多效细胞因子。通过分子克隆得到了斜带石斑鱼Epinephelus coioidesTGF β1 cDNA全长序列，全长为2 477 bp，开放阅读框为1 161 bp，编码一种含386个氨基酸的多肽。Realtime PCR分析表明，TGF β1基因在健康斜带石斑鱼所取组织中均有表达，肾脏中表达最高，其次是头肾和脾脏。用PolyI:C或ConA刺激斜带石斑鱼头肾淋巴细胞，TGF β1表达量明显上升，且在刺激4 h后达到最高值。用PolyI:C刺激16、24 h后，TGF β1几乎不表达，而用ConA刺激实验组，TGF β1表达量一直高于对照组。结果说明，斜带石斑鱼TGF β1基因结构和表达特征与其他硬骨鱼类具有高度相似性。

Molecular Cloning and Expression Analysis of Transforming Growth Factor-β1(TGF-β1)from Orange-Spotted Grouper(Epinephelus coioides)

Transforming growth factor β1 (TGF β1) is a highly conserved pleiotropic cytokine. In this study, we presented the bioinformatics analysis of TGF β1 full length cDNA sequences from orange spotted grouper (Epinephelus coioides). The full length cDNA is 2477 bp, which contains an ORF of 1161 bp, encoding a 386 amino acids protein. Real time PCR results showed that TGF β1 mRNA was predominantly expressed in the kidney, followed by head kidney and spleen of the healthy orange spotted grouper. The expression of TGF β1 in isolated headkidney lymphocytes was up regulated treated with PolyI:C or ConA, and peaked at 4 hours after stimulation. Lymphocytes groups which were treated with PolyI:C had little TGF β1 expression after 16 and 24 hours, while the expression of TGF β1 was significantly higher than the control groups during the whole experimental processing time treated with ConA. In conclusion, we found the structure and expression pattern of grouper TGF β1 was similar to those of other fish.