| 棒状杆菌表达载体pJL23的构建 |
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| 引用本文： | 陆军,唐炯,张文波,严维耀,郑兆鑫.棒状杆菌表达载体pJL23的构建[J].复旦学报(自然科学版),2000,39(3):292-296. |
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| 作者姓名： | 陆军 唐炯 张文波 严维耀 郑兆鑫 |
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| 作者单位： | 1. 常熟高等专科学校生物系,常熟 215500
2. 生命科学学院,遗传工程国家重点实验室 |
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| 摘 要： | 质粒ｐＧＡ４６－４带有从谷氨酸棒杆菌染色体上分离到的有启动功能的片段，用ＰＣＲ技术从ｐＧＡ４６－４中扩增了该片段的关键区域；启动子ＰＧＬ，将该启动子经ＥｃｏＲⅠ－ＢａｍＨ Ⅰ双酶切后，与大肠杆菌质粒ｐＪＬ０１的ＥｃｏＲⅠ－ＢａｍＨⅠ大片段连接，再接入Ｘｙｌ Ｅ ｇｅｎｅ和棒状杆菌质粒ｐＸＺ１０１４２，构建成棒状杆菌－大肠杆菌穿梭表达载体ｐＪＬ２３。用邻苯二酚双加氧酶基因检测表明，该表达载体可在棒状
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| 关 键 词： | 启动子 pXZ10142 pJL23 棒状杆菌 克隆表达载体 |
| 文章编号： | 0427-7104(2000)03-0292-05 |
Construction of a Expression Vector pJL23 in Corynebacteria |
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| LU Jun,TANG Jiong,ZHANG Wen-bo,YAN Wei-yao,ZHENG Zhao-xin.Construction of a Expression Vector pJL23 in Corynebacteria[J].Journal of Fudan University(Natural Science),2000,39(3):292-296. |
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| Authors: | LU Jun TANG Jiong ZHANG Wen-bo YAN Wei-yao ZHENG Zhao-xin |
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| Institution: | LU Jun
,TANG Jiong
,ZHANG Wen-bo
,YAN Wei-yao
,ZHENG Zhao-xin
(State Key Laboratory of Genetic Engineering, School of Life Sciences) |
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| Abstract: | Plasmid pGA46 4 contains a promoter function fragment isolated from the chromosome of Corynebacterium glutamicum . The essential part of this fragment promoter P GL is amplified from pGA46 4 by PCR. In order to construct a shuttle expression plasmid pJL23, this promoter was ligated with Escherichia coli plasmid pJL01, the Xyl E gene and the corynebacteria plasmid pXZ10142. According to the Catechol 2,3 dioxygenase expression results, the expression vector functions well in the expression of foreign genes in corynebacteria. |
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| Keywords: | promoter PCR Xyl E gene pXZ10142 |
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